Catalog :

Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 50 / No. 1 / Published : 2014-01

TOC : 3, and page :10 - 14

Related with : Scholar   Yahoo!   Bing

Original Article :

Agreement between estimated apolipoprotein b-100 concentration and apolipoprotein b-100 concentration measured by immunoturbidimetric method

Author :

  1. Hadisiswoyo P*1
  2. Soehita S*2
  3. Anniwati L*3
  1. Resident at the Department of Clinical Pathology, Faculty of Medicine, Airlangga University, Surabaya
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

Apo B-100 is synthesized in the liver and only one apo B molecule exists in every LDL, IDL and VLDL particle, therefore, the level ofapo B-100 represents the total number of potentially atherogenic lipid. A formula estimating apo B-100 might help clinicians inareas with limited resources to identify patients with risk factors for cardiovascular disease, so proper management can be carriedout. To evaluate an agreement between estimated apolipoprotein B-100 concentration and apolipoprotein B-100 concentrationmeasured by immunoturbidimetric method. This study was an analytical observational cross sectional design. Serum samples werecollected using simple random sampling method and all specimens were measured for LDL-cholesterol, triglyceride andapolipoproten B-100 concentration. Estimated apo B-100 concentrations were counted using a [Apo B-100= -33.12 + 0.675 x LDL+ 11.95x ln (TG)] formula. Twenty nine of 30 samples (96.67%) showed estimated apo B-100 concentrations higher than measuredapo B-100 concentrations. Difference of the mean of estimated and measured apo B-100 concentration was 10.941 mg/dL, and wasstatistically significant (t= 9.134 (p <0.0001)). Concordance correlation coefficient (CCC) between estimated and measuredapolipoprotein B-100 concentration was 0.854 (<0.90= poor agreement). The agreement between estimated apo B-100concentration and apo B-100 concentration measured with immunoturbidimetric method was poor. Factors that might have causedthis result were freeze-thawing of samples prior to apo B-100 measurement using immunoturbidimetry and the use of triglyceride asone of the variables in the formula in estimating apo B-100.(FMI 2014;50:10-14)

Keyword :

Apo B-100, LDL-cholesterol, triglyceride, immunoturbidimetry,

References :

  1. Brown SA, Epps DF, Dunn JK, Sharrett AR, Patsch JR, Gotto AM Jr, Patsch W, (1990). Effect of blood collection and processing on radioimmunoassay results for apolipoprotein B in plasma. - : Clin Chem
  2. Brunzell JD, Davidson M, Furberg CD, Goldberg RB, Howard BV, Stein JH, Witztum JL, (2008). Lipoprotein management in patients with cardiometabolic risk. - : J Am Coll Cardiol


Archive Article

Cover Media Content

Volume : 50 / No. : 1 / Pub. : 2014-01
  1. Genetic mutation profile of gyra and parc gene in ciprofloxacin-resistance pseudomonas aeruginosa
  2. The depression profile of people living with hiv/aids (plwha) receiving antiretroviral treatment in dr. soetomo hospital surabaya
  3. Agreement between estimated apolipoprotein b-100 concentration and apolipoprotein b-100 concentration measured by immunoturbidimetric method
  4. Er, pr, and her-2/neu profile on young breast carcinoma patients
  5. Correlation between heart type fatty acid binding protein serum level and soluble st2 in acute coronary syndrome patients
  6. Correlation between n-terminal pro-brain natriuretic peptide level with dyastolicfunction echocardiography parameter in acute coronary syndrome
  7. Correlation between e/vp and e/e’ in left ventricular filling pressure measurement among patients with dilated cardiomyopathy
  8. Changes in platelet count, mean platelet volume, platelet distribution width, and plateletcrit in pulmonary tuberculosis severity
  9. Microbial pattern and antibiotic susceptibility test in semen gresik hospital 2012
  10. Diagnostic values of immature granulocytes, eosinopenia and i/t ratio in detection of early onset neonatal sepsis in neonates with bacterial infection risk
  11. Effect of coffee drinks to the reaction time of 18-22 years old male university students
  12. Influence of weightlifting on the emergence of partial and full thickness rotator cuff tear detected by ultrasound imaging
  13. Mean score of cervical pedicle angle to midline and inferior end plate in adult using multi sliced computed tomography