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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 50 / No. 1 / Published : 2014-01

TOC : 3, and page :10 - 14

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Original Article :

Agreement between estimated apolipoprotein b-100 concentration and apolipoprotein b-100 concentration measured by immunoturbidimetric method

Author :

  1. Hadisiswoyo P*1
  2. Soehita S*2
  3. Anniwati L*3
  1. Resident at the Department of Clinical Pathology, Faculty of Medicine, Airlangga University, Surabaya
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

Apo B-100 is synthesized in the liver and only one apo B molecule exists in every LDL, IDL and VLDL particle, therefore, the level ofapo B-100 represents the total number of potentially atherogenic lipid. A formula estimating apo B-100 might help clinicians inareas with limited resources to identify patients with risk factors for cardiovascular disease, so proper management can be carriedout. To evaluate an agreement between estimated apolipoprotein B-100 concentration and apolipoprotein B-100 concentrationmeasured by immunoturbidimetric method. This study was an analytical observational cross sectional design. Serum samples werecollected using simple random sampling method and all specimens were measured for LDL-cholesterol, triglyceride andapolipoproten B-100 concentration. Estimated apo B-100 concentrations were counted using a [Apo B-100= -33.12 + 0.675 x LDL+ 11.95x ln (TG)] formula. Twenty nine of 30 samples (96.67%) showed estimated apo B-100 concentrations higher than measuredapo B-100 concentrations. Difference of the mean of estimated and measured apo B-100 concentration was 10.941 mg/dL, and wasstatistically significant (t= 9.134 (p <0.0001)). Concordance correlation coefficient (CCC) between estimated and measuredapolipoprotein B-100 concentration was 0.854 (<0.90= poor agreement). The agreement between estimated apo B-100concentration and apo B-100 concentration measured with immunoturbidimetric method was poor. Factors that might have causedthis result were freeze-thawing of samples prior to apo B-100 measurement using immunoturbidimetry and the use of triglyceride asone of the variables in the formula in estimating apo B-100.(FMI 2014;50:10-14)

Keyword :

Apo B-100, LDL-cholesterol, triglyceride, immunoturbidimetry,


References :

  1. Brown SA, Epps DF, Dunn JK, Sharrett AR, Patsch JR, Gotto AM Jr, Patsch W, (1990). Effect of blood collection and processing on radioimmunoassay results for apolipoprotein B in plasma. - : Clin Chem
  2. Brunzell JD, Davidson M, Furberg CD, Goldberg RB, Howard BV, Stein JH, Witztum JL, (2008). Lipoprotein management in patients with cardiometabolic risk. - : J Am Coll Cardiol


   


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Volume : 50 / No. : 1 / Pub. : 2014-01
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