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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 48 / No. 3 / Published : 2012-07

TOC : 6, and page :121 - 125

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Original Article :

Benzalkonium chloride and pine oil-containing cleaning fluid is not effective against pseudomonas aeruginosa

Author :

  1. Lembah Sulistyaningsih*1
  2. Eko Budi Koendhori*2
  3. Ramadhani*3
  1. Mahasiswa Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

Nosocomial infection is one of the leading cause of the increasing of morbidity and mortality in the hospital. Pseudomonas aeruginosa is included in one of the most three bacteria that cause opportunistic infections in human including nosocomial infections. WHO in 2002 has issued guidelines prevention of nosocomial infections, including infections by Pseudomonas aeruginosa. One preventive measure is to maintain a clean hospital environment, both macroscopically and microscopically. One of the measures to prevent nosocomial infection is a clean hospital environment by using materials routinely cleaning floors, walls, bathrooms, etc. Among the many brands of floor cleaner ingredients antimicrobial active substances most widely used is benzalkonium chloride and pine oil. Therefore the researcher are examining the effectiveness of floor cleaning fluid which contain benzalkonium chloride and pine oil. The objective of this study was to know the effectiveness comparison between the floor cleaner fluid which is containing antimicrobial substance benzalkonium chloride and pine oil against Pseudomonas aeruginosa.This research is true experimental research which is done diffusion modification method in the microbiology laboratory. Floor cleaner fluid being used is floor cleaning agents containing 450 ml of 1.5% benzalkonium chloride which was then diluted in accordance with the rules of usage indicated on the packaging and floor cleaning agents containing 450 ml of 2.5% Pine oil is then diluted in accordance with the rules the use indicated on the packaging. Then the floor cleaning materials are placed on wells that have been made on agar plates that had been planted with Mueller Hinton bacterium Pseudomonas aeruginosa with streaking method. In order for the plate and then put into an incubator, and observed the formation of bacterial growth inhibition zones around the wells. Results revealed that no bacterial growth inhibition zone was formed around the wells which has given floor cleaning fluid therein. In conclusion, the floor cleaning fluid which is containing antimicrobial substance whether benzalkonium chloride or pine oil are not effective againts Pseudomonas aeruginosa. (FMI 2012;48:121-125)

Keyword :

floor cleaning fluid, Pseudomonas aeruginosa, benzalkonium chloride, pine oil, diffusion,


References :

  1. Brooks GF, Butel JS, Morse SA, (2005). Jawetz, Melnick & Adelbergh’s: Mikrobiologi Kedokteran, 1st ed. Jakarta : Salemba Medika
  2. Conroy BP, Anglen JO, Simpson WA, Christensen G, Phaup G, Yeager R, Gainor BJ, (1999). Comparison of castile soap, benzalkonium chloride, and bacitracin as irrigation solutions for complex contaminated orthopaedic wounds. - : J Orthop Trauma


   


Archive Article

Cover Media Content

Volume : 48 / No. : 3 / Pub. : 2012-07
  1. In vitro intraerythrocytic antimalarial activity of akar kuning (arcangelisia flava (l.) merr.) stem aqueous extract in plasmodium falciparum
  2. In vitro antimalarial activity of belimbing wuluh (averrhoa bilimbi) leaves extract on plasmodium falciparum
  3. Effect of earthworms (lumbricus sp.) extract antibacterial activity against the bacteria salmonella typhii
  4. In vitro antibacterial activities of commercial probiotic drinks against salmonella typhii
  5. Essential oil extract of citrus aurantifolia l. has better antibacterial effect than sulfur towards staphylococcus epidermidis
  6. Benzalkonium chloride and pine oil-containing cleaning fluid is not effective against pseudomonas aeruginosa
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