UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Agro Veteriner

ISSN 2303-1697

Vol. 3 / No. 1 / Published : 2014-12

Order : 8, and page :56 - 64

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Original Article :

Detection of flaa gene thermotolerant campylobacter jejuni isolated from chicken meat using polymerase chain reaction

Author :

  1. Winda Ardianti*1
  2. Rahayu Ernawati*2
  3. Soeharsono*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This study was aimed to detect one of virulence factor, flagellin A (flaA) of C. jejuni on chicken meat sold in traditional market by using Polymerase Chain Reaction (PCR) method. Twelve chicken legs obtained from traditional markets were collected, diluted in Broth Pepton Water, enrichment in Preston broth, and it was then cultured on the modified charcoal cefoperazone deoxycholate agar (mCCDA). Colonies on this medium were harvested and DNA was extracted. Supernatant from DNA extraction was separated from pellet and used as DNA template. PCR amplification was run in DNA thermal cycler on a total volume of 20 µl with annealing temperature at 58ºC for 1 min. PCR products were analyzed using electrophoresis method. The results showed two samples had a band of the 650 bp of 23S rRNA gene, which means that chicken meat samples contain thermotolerant Campylobacter sp. One sample had a band of the 1558 bp of flaA gene, meaning one of C. jejuni that contaminated chicken meat samples contain virulence factors.

Keyword :

flaA gene, 23S rRNA gene, Campylobacter jejuni, chicken meat, Polymerase Chain Reaction,


References :

Andriani, S. M. Noer, M. Poeloengan, dan Supar,(2006) Pengembangan Enzyme–Linked Immuno Sorbent Assay untuk Deteksi Antigen Campylobacter jejuni pada Daging Ayam bogor : Balai Besar Penelitian Bogor





Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-12
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  2. Ibm Kelompok Ternak Sapi Perah Di Kecamatan Bantur Kabupaten Malang
  3. Financial And Sensitivity Analysis Of Intensive Vannamei Shrimp Cultivating In Subdistrict Of Panceng, Gresik Regency
  4. Hubungan Sistem Manajemen Proses Produksi Terhadap Analisa Usaha Peternakan Ayam Petelur Jantan Di Kabupaten Malang
  5. Crude Protein Digestibility Value Of Complete Feed In Rat (rattus Norvegicus)
  6. Pemberian Probiotik Asam Laktat Dalam Air Minum Terhadap Berat Badan Akhir Dan Persentase Karkas Pada Ayam Broiler Strain Hubbard Umur 35 Hari
  7. Ibm Kelompok Ternak Sapi Potong Dan Kelompok Tani Kedelai Kabupaten Lamongan Dalam Pemenuhan Swasembada Daging
  8. Detection Of Flaa Gene Thermotolerant Campylobacter Jejuni Isolated From Chicken Meat Using Polymerase Chain Reaction

Information FMI@comparison-of-apoptotic-index-and-the-amount-of-brain/AGROVET