UNIVERSITAS AIRLANGGA



Detail Article

BIOSAINS

ISSN 1412-1433

Vol. 13 / No. 1 / Published : 2011-01

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Original Article :

Bioactivity human menopause gonadotrophin (hmg) and hmg deglycosylated (hmgdg) of urine of postmenopause woman toward cleavage embryo of cattle in vitro

Author :

  1. Herry Agoes Hermadi*1
  2. L. Mahaputra*2
  3. Agus Abadi*3
  1. Departemen Reproduksi, Fakultas Kedokteran Hewan, Universitas Airlangga
  2. Departemen Reproduksi, Fakultas Kedokteran Hewan, Universitas Airlangga
  3. Departemen Obgin Fakultas Kedokteran, Universitas Airlangga

Abstract :

The aim of this study was to produce hMG from the urine of post-­menopausal women and to evaluate the influence of glycan removal from hMG glycoprotein molecule undergoing deglycocylation (hMGdG) in shortening the onset of cleavage embryo of bovine in vitro. The experiments were performed on: 1) the identification of hMG from the urine of postmenopausal women by confirming the glycoprotein characteristic; 2) the examination of biochemical characteristic of hMG deglycosylated using N-glycanase; 3) the influence of deglycosylated hMG on the onset of embryo of bovine cleavage in vitro.Urine samples were collected from 30 postmenopausal women. The result of SDS-PAGE demonstrated protein bands with molecular weight ranging from 19.4–107 kDa. Western Blot using anti-hMG monoclonal antibody (Catalogue CSA-614 Stressgen Bioreagents) showed that immunoreactivity at the band with molecular weight of 30 kDa which was a glycoprotein. The band result of Western blot referred with MW 30 kDa was glycoprotein. Glycoprotein is a protein molecule attached to carbohydrate molecule by using Glycoprotein Carbohidrat Estimation Kit 23260. The concentration rate of glycoprotein was 99860.00 μg/ml (PAS), protein was 66939.29 μg/ml (Biuret) and carbohydrate 32920.71 μg/mL (PAS). Concentration ratio of glycoprotein, protein and carbohydrate from molecule hMG was3: 2: 1. hMG from the urine of the 30 postmenopausal women was confirmed with the 30 kDa molecular weight glycoprotein characteristic. The Chemical characteristic of hMG undergoing enzymatic deglycosylation using N-glycanase reduced the molecular weight into 26 kDa. The deglycocylated hMG reduced the onset of cleavage embryo of bovine in vitro from 24 to 20 hours (p < 0.01).

Keyword :

hMG, hMGdG, embryo cleavage, glycoprotein, deglycocylation,


References :

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Archive Article

Cover Media Content

Volume : 13 / No. : 1 / Pub. : 2011-01
  1. Expression Of P53 In Progresive And Non Progresive Type Of Pterygium By Human Papilloma Virus Type 18 Infection
  2. Bioactivity Human Menopause Gonadotrophin (hmg) And Hmg Deglycosylated (hmgdg) Of Urine Of Postmenopause Woman Toward Cleavage Embryo Of Cattle In Vitro
  3. Extracellular Signal Regulated Kinase (erk1/2), Reseptor Estrogen (er) A, And Insuline Like Growth Factor I (igf-ir) Expressions On Osteoblast In Influencing Bone Density In The Female Young Rat (rattus Norvegicus) After Exercise Training
  4. The Role Of Endonuclease-g For Amniotic Cell Apoptosis Biomarker Determination In Pregnancies With Premature Rupture Of The Membrane
  5. The Microdeletion Of Rbm And Daz Genes In Male Partner Of Infertile Couple In Population With Inbreeding
  6. Compartion Study Between Animal Model Sopk And Normally Clycle Is Mmp-9, Timp-1 Enzym Activities And Collagen Expression (an Experiment Study In Rat)
  7. Characterization And Isolation Of Specific Protein From Excretory-secretory (es) Material Of L2 Dormant Of Toxocara Cati For The Diagnostic Development Of Toxocariasis By Elisa Tehnique

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