UNIVERSITAS AIRLANGGA



Detail Article

BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 2 / Published : 2012-05

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Original Article :

The analyze of human dna soft tissue that contaminated formalin during 1 month and 6 month at locus d13s317 using str-pcr

Author :

  1. Arlene Kusumadewi *1
  2. Soekry Erfan Kusuma*2
  3. Ahmad Yudianto*3
  1. Universitas Hang Tuah Surabaya
  2. Fakultas Kedokteran Universiats Airlangga
  3. Fakultas Kedokteran Universiats Airlangga

Abstract :

Dead body preservation by using formalin 10% injection is a procedure that has been laid down in medical institution to preservate theabandon debody who’s still intact before used as a research object. The similar thing was also been done because of recent reason, forinstance his or her family wants to keep the dead body more than 24 hours before it is buried or being cremation, therefore, in orderto safe the environment, the dead body should be righteously preservated. Objective of this study was to analysis of formalin impact6 month until 1 month interval against human soft tissue DNA. To the effect of observational it is subject to be menganalisis formalpresentation infl uence with time interval 1 month up to 6 months to DNA human soft network as material as DNA’S check at forensicarea. This observational type is experimental laboratoris with DNA’S sample indigenous core a part human soft network muskuluspsoas major that is taken from one jenasah abandon at Forensic Medical Science Installation RSUD Dr. Soetomo Surabaya. Analisis’sresult points out to get a long time formal presentation that is given on human soft network sample, therefore available DNA’S rate trendthat gets menurun but still lies on minimal sill point needed DNA rate on check Short is Repeat’s Tandem (STR). DNA’S visualisationresult on FGA’S locus, D13S317, D18S51 and D21S11 in observational it all still gets to be detected. Decrease titrates DNA on humansoft network one most formal fl at that not evoke meaning effect, that cause DNA human soft network that its ability loss as materialas DNA’S identifi cation forensic.

Keyword :

Human soft tissue DNA, , formalin expossure, , STR-PCR,


References :

Atmadja DS dan Untoro E,(2008) Peranan Analisis DNA pada Penanganan Kasus Forensik, Jakarta : Penerapan Ilmu Kedokteran Forensik dalam Proses Penyidikan,

Evans and Thomas C,(2007) DNA Damage, The Major Cuse of Missing Pieces From the DNA Puzzle, New England : New England Biolabs Inc

Kusuma SE dan Yudianto A,(2010) Forensik Molekuler, dalam Buku Ajar Ilmu Kedokteran Forensik dan Medikolegal, edisi keenam, Ed. Hariadi A, Hoediyanto. Surabaya : Departemen Ilmu Kedokteran Forensik dan Medikolegal Fakultas Kedokteran Universitas Airlangga

Sosiawan A,(2007) Analisis efek paparan panas suhu ekstrim tinggi terhadap DNA yang berasal dari tulang dan gigi. Surabaya : Program Pascasarjana Universitas Airlangga

Wilianto W,,(2010) Pengaruh Paparan Formalin Secara Prosentase Terhadap DNA Muskulus Psoas Mayor dengan Pemeriksaan Metode PCR Lokus CSF1P0, D5S818, D13S317, D21S11, Surabaya. : Dept/Inst. Ilmu Kedokteran Forensik dan Medikolegal FK Unair- RSUD Dr. Soeto



Archive Article

Cover Media Content

Volume : 14 / No. : 2 / Pub. : 2012-05
  1. Analysis Of Myoc Gene Polymorphism Among Malay Race Patients With Primary Open Angle Glaucoma In Palembang
  2. Polymorphism Analysis Of Alpha–helix Coiled Coil Rod Homologue Gene Exon 2 To Patients With Psoriasis In Palembang Malay Race
  3. Isolation And Identification Of High Cellulolytic Activity Of Bacteria From Golden Snail’s (pomacea Canaliculata) Gut
  4. Biocompability Of Low Dose Of Human Menopause Gonadotropin(hmg) And Deglycosilation Of Human Menopause Gonadotropine (dghmg) Against Bovine Eggs
  5. Profile Of Narcotic Evidence From Black Circulation Market In Law Police Area East Java Using Gc-ms Method
  6. Analysis The Influence Of Time And Detergen Washing To Dna From Cement Liquids Spotted In Fga Locus, D21s11 And Dys19 Using Str-pcr Method
  7. The Analyze Of Human Dna Soft Tissue That Contaminated Formalin During 1 Month And 6 Month At Locus D13s317 Using Str-pcr

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