UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Medical Microbiology

ISSN

Vol. 1 / No. 1 / Published : 2012-12

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Original Article :

Ziehl neelsen stain sensitivity in determining the etiologic of granulomatous inflammation

Author :

  1. Wilda Mahdani*1
  2. Ni Made Mertaniasih*2
  3. Arthur Pohan Kawilarang*3
  4. Troef Soemarno*4
  1. Department of Microbiology, Medical Faculty of Syiah Kuala University, Darussalam
  2. Department of Microbiology, Faculty of Medicine, Airlangga University, Surabaya
  3. Department of Microbiology, Faculty of Medicine, Airlangga University, Surabaya
  4. Department of Pathology, Faculty of Medicine, Airlangga University, Surabaya

Abstract :

The main infectious agent of granulomatous inflammation is Mycobacterium tuberculosis. There are  a lot of forms and patterns of tuberculosis infection. Clinicians are generally more suspicious of pulmonary infection, while infection of tuberculosis in the other tissue is often overlooked. This observational descriptive study aimed to find out the sensitivity of Ziehl Neelsen (ZN) stain in determining etiologic diagnosis of granulomatous inflammation on histopathological specimens. Study samples were 37 pieces tissue blocks which diagnosed as granulomatous inflammation. The incidence of mycobacteria infection causes granulomatous inflammation in the sample studied was 62.16%. The study found positive Acid Fast Bacilli (AFB) in 23 tissue block. The conclusion of this study was ZN staining technique had 81% sensitivity, 90% specificity, 96% Positive Predictive Value (PPV) and  64% Negative Predictive Value (NPV) to determine the etiologic diagnosis of  specific granulomatous inflammation. Special staining techniques can be used to diagnose the etiology of tissue infection that will result in appropriate therapy for patients.

Keyword :

Granulomatous inflammation, Specific granuloma, Ziehl-Neelsen Staining,


References :

Brancroft, JD,(2002) Theory and Practice of Histological Techniques London : Churchill Livingstone





Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2012-12
  1. Antimicrobial Activity Of Hibiscus Sabdariffa Calyx Extract Against Shigella Dysenteriae In Vitro
  2. Bactericidal Effect Of Cempedak (artocarpus Champeden) Stem Extract On Mycobacterium Tuberculosis
  3. Antibacterial Activity Of Papaya Leaves (carica Papaya,l) Infusum Against Escherichia Coli
  4. Ziehl Neelsen Stain Sensitivity In Determining The Etiologic Of Granulomatous Inflammation
  5. Comparative Antibacterial Activity Of Noni Fruit Versus Ciprofloxacin Against Salmonalla Typhii In Vitro
  6. Susceptibility Pattern Of Mycobacterium Tuberculosis From Bronchoalveolar Lavage (bal) Specimen

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