UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 2 / No. 2 / Published : 2010-11

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Original Article :

Kultur sel otak ikan kerapu bebek (cromileptes altivelis) dengan menggunakan media l-15, mem dan tcm

Author :

  1. Hari Suprapto*1
  2. Nunik Diantiwi *2
  1. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo - Surabaya
  2. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo - Surabaya

Abstract :

 Humpback grouper is high value commodity fish. The demand of domestic and foreign markets increases every year. The obstacles of Humpback grouper fish cultivation is a disease caused by Viral Nervous Necrosis  (VNN). The virus attacks the brain leading to mass death. To prevent the disease, a research on VNN virus has to be started. The problem of the research is the lack of culture of fish tissue. Virus have specific location to tissue cell and species to be infected. Thereofore, first step must be done is growth from tissue cell which infection of virus. Cell culture is a technique to isolate cell, protoplasm, tissue or organ to be grown in a sterile condition with nutrition containing growth hormone to promote multiplication. Cell culture technique can be used in many basic applications in cell biology. The purpose of this research is to develop cell culture from Grouper fish brain for VNN research. The hope is culture can grow and develop well.Research executed at June until Desember 2009 in Laboratory In Vitro of Veterinari Faculty of Airlangga University and Laboratory Gastroenteritis Institute of Tropical Disease, Airlangga University, Surabaya. The result showed that brain cell culture of humpback grouper fish can be grow and multiply well in all three media, media L-15 cell growth was faster compared to media MEM and TCM.

Keyword :

Humpback grouper fish, VNN, Cell culture, Medium L-15, MEM, TCM ,


References :





Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2010-11
  1. The Use Of Sand Substrate As Media For Tiger Shrimp (penaeus Monodon) Post Larva Rearing
  2. The Production Of Superoxide From Shrimp Penaeus Monodon Hemocyte As An Stress Indicator On The Intensive Culture
  3. Isolation Of Aeromonas Hydrophila From Diseased Shrimp Penaeus Monodon Cultured In Traditional Ponds
  4. Efek Ph Pada Air Pemeliharaan Ikan Mas Cyprinus Carpio Terhadap Pertumbuhan Bakteri Aeromonas Hydrophilla
  5. Penambahan Ekstender Madu Dalam Proses Penyimpanan Sperma Beku terhadap Motilitas Dan Viabilitas Spermatozoa Ikan Komet (carassius auratus Auratus)   
  6. Penentuan Faktor Pengelolaan Tambak Yang Mempengaruhi Produktivitas Tambak Kabupaten Mamuju, Provinsi Sulawesi Barat
  7. Kultur Sel Otak Ikan Kerapu Bebek (cromileptes Altivelis) Dengan Menggunakan Media L-15, Mem Dan Tcm
  8. Efektivitas Penggunaan Whole Cell Dari Vibrio Alginolyticus Sebagai Vaksinoral Melalui Artemia Pada Benih Ikan Kerapu Tikus (chromileptes Altivelis)
  9. Isolasi Bakteri Indigen Sebagai Pendegradasi Bahan Organik Pada Media Pembenihan Ikan Lele Dumbo (clarias Sp.) Sistem Resirkulasi Tertutup
  10. Perubahan Patologi Kulit Ikan Gurami (osphronemu Gouramy) Akibat Infestasi Lernaea Cyprinacea Pada Derajat Infestasi  Yang Berbeda
  11. Pengaruh Pemberian Berbagai Jenis Pakan Terhadap Pertumbuhan Dan Tingkat Kelulushidupan Ikan Silih (macrognathus Aculeatus)
  12. Kemampuan Reproduksi Daphnia Magna Jantan Hasil Induksi Logam Berat (cd, Pb) Dan Pestisida Diazinon
  13. Penambahan Suplemen Madu Dalam Pakan Guna Meningkatkan Pertumbuhan Dan Kelulushidupan Benih Lobster Air Tawar Red Claw (cherax  Quadricarinatus)
  14. Sargassum Sp. Sebagai Biokontrol Terhadap Kandungan Logam Berat Timbal (pb) Yang Terserap Oleh Kerang Darah (anadara Granosa)
  15. Efektifitas Bakteri Pseudomonas Sebagai Pengurai Bahan Organik (protein,karbohidrat,lemak) Pada Media Air Limbah Pembenihan Ikan Lele Dumbo (clarias Sp.) Sistem Resirkulasi Tertutup
  16. Obyek Kesukaan Untuk Penempelan Telur (oviposisi)  Ektoparasit        Argulus Japonicus
  17. Kondisi Muara Porong Berdasarkan Indeks Klorofil-a  Dan Total Suspended Solid (tss)
  18. Pengaruh Pemberian Beberapa Bakteri Terhadap Kelangsungan Hidup Benih Ikan Lele Dumbo (clarias Sp)
  19. The Effect Of Using Kombucha On Crude Protein And Crude Fiber Contents In Water Hyacinth (eichornia Crassipes) Fermentation

Information FMI@comparison-of-apoptotic-index-and-the-amount-of-brain/JIPK