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Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

Order : 1, and page :227 - 245

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Original Article :

Genotyping viral nervous necrosis (vnn) in groupers in east java and west nusa tenggara

Author :

  1. Devy Rahmawati Putri*1
  2. Suwarno*2
  3. Gunanti Mahasri*3
  1. Mahasiswa Pasca Sarjana
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Pasca Sarjana

Abstract :

The general objective of the research are to characterize genotypes of Viral Nervous Necrosis (VNN) and analyze kinship genotypes on grouper in East Java and West Nusa Tenggara. The samples of this research were taken from the fish center of the cultivation of various regions, such as Situbondo region, Banyuwangi, Gresik, Tuban, West Lombok, East Lombok and Sumbawa. This research is divided into two stages, the first step is by inspecting of grouper which are indicated VNN infection with the tecnique of conventional PCR and the second step is following by amplification using specific primer coat protein and DNA sequensing. After that followed by homology analysis and then do a comparison with the data of China RGNNV EF558369 GenBank and Bali HQ859926.1. From the result of research shows that the point mutation at nucleotide base pair which has been compared to the data from China RGNNV EF558369 GenBank and Bali HQ859926.1. The Kinship analysis is based on the nucleotide composition between regions which has the same kinship that is included in the genotype Red-spotted Grouper Nervous Necrosis Virus (RGNNV) in which after the comparison between regions have similarities ranging from 9593.53%-98.63% with the data GenBank GenBank Gondol BALI HQ859926 and EF558369.1 RGNNV China. Phylogenetic analysis based on the grouping of each sample seen that each area has in common with the genotype of the reference data RGNNV Isolate china and different from the other genotypes. This analysis serves to determine kinship because of the distribution of seeds from one area to another.

Keyword :

Viral Nervous Necrosis, Genotyping, ikan kerapu,


References :

Yang, H. L.,(2007) Fish Oral Vaccine That Can Induce Protective Immunity at the Early Post-Larvae Stage of Grouper 1: 07 : Institute of Biotechnology

Nishizawa, T., K. Mori, M. Furuhashi, T. Nakai, I. Furusawa, and K. Muroga,(1995) Comparison of The Coat Protein Genes of Five Fish Nodaviruses, the Causative Agents of Viral Nervous Necrosis in Marine Fish 76 : 1563-1569 : Journal of General Virology

Nakai, T., K. Mori., T. Sugaya, T. Nishioka, K. Mushiake, H. Yamashita,(2009) Current knowledge on viral nervous necrosis (VNN) and its causative betanodaviruses 61: 198–207 : Isr. J. Aquacult. Bamid





Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping Viral Nervous Necrosis (vnn) In Groupers In East Java And West Nusa Tenggara
  2. Lactoferrin Effect On Biofilm Formation Of Klebsiella Pneumoniae Isolated From Mastitis Cases Of Dairy Cows
  3. Effect Of Insulin Transferrin Selenium (its) Supplementation In Maturation Medium Tcm-199 To Bovine Oocyte Diameter
  4. Supplementation Of Insulin Transferrin Selenium On In Vitro Maturation Cumulus Oocyte Complex (coc) Against To Mitogen Activated Protein Kinase (mapk) Expression And Expanded Cumulus Mass
  5. Adaptation Capability And Antigen Stability Of Newcastle Disease Virus Local Isolate On Hela Cell
  6. Comparison Of Antibody Titre Of Anti Brucella Abortus Using Cft And Ielisa To Cattle With Positive Rbt
  7. Potency Of Apple Vinegar As Alternatif Therapy Against Wound Healing Time Of Staphylococcus Aureus Infection On Mice
  8. Suplementation Of Specific Protein Membrane Spermatozoa In Freezing Medium To Improvement Efforts A Succes Cattle Frozen Semen Quality Post Thawing
  9. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat After Infected By Salmonella Typhimurium
  10. The Potential Of Outer Membrane Protein Brucella Abortus Local Isolate As Brucellosis Vaccine Development In Indonesia
  11. The Using Of Whole Serum Pmsg (pregnat Mare Serum Gonadotropin) With Sepadex Extracted For Increasing Estrus Induction Of Ettawa Goat
  12. Toxicity Test Of Singawalang (petiveria Alliaceae) Leaves Extract To The Hypertrophy Of Male White Mice (mus Musculus) Cardiac Muscle
  13. Effect Of Benzo(a)pyrene Injection To The Initiation Of Mammae Cancer At Mice

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