UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 1 / Published : 2008-02

Order : 15, and page :73 - 78

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Original Article :

Protein characterization of excretory secretory second stage larvae dorman against antibody anti-second stage larva toxocara canis by western blot techniques

Author :

  1. Laula Putri Damia*1
  2. Setiawan Koesdarto*2
  3. M. Gandul Atik*3
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Toxocariasis is caused by Toxocara canis, Toxocara cati and other Toxocara species, which are intestinalnematodes (roundworms) found in dogs and cats. Human are incidental host, and the parasites cannot completelymature in the human body. Three clinical forms of toxocariasis are described : these include viscerallarvae migrans (VLM), ocular larvae migrans (OLM), and covert toxocariasis. Toxocariasis is a disease whoseclinical manifestation are unspecific, characterizied by larvae migration to inner organs of human and someanimal. In the absence of parasitological evidence of infection, immunological methods are required for itsdiagnosis. The identification of specific antigens of Toxocara canis is important in order to develop betterdiagnostic techniques. Fourteen mouse were infected orally with a dose of 850 of Toxocara canis embryonatedeggs. Mouse were bled periodically to collect the antibody. Western blot (WB) assay was perfomed usingexcretory secretory antigens (E-S) of T. canis second stage larvae dorman. Mouse sera were diluted 1:50.secondary antibody was used at a dilution of 1:1000. The result of the study indicated eight protein bands,which are 62,4 kDa; 46,4 kDa; 43,1 kDa; 37,3 kDa; 30,1 kDa; 24,4 kDa; 19,4 kDa and 10,7 kDa by westernblot techniques These protein need further evaluation as candidate for use in diagnosis.

Keyword :

Toxocara canis, Western blot, Toxocariasis, ., .,


References :

Abbas, A. K., A. H. Litchman and Pober,(2000) Cellular and Mollecular Immunologi Philadelphia, USA : Saunders Company

Abdel-Rahman, E. H., K. N. Abdel-Megged and M. A. Hassanain,(2000) Structural Characterization and Immunocatalization of Egg Antigen Cross- React with Toxocara vitulorum, Fasciola gigantica and Moniezia expanza . : J. Egypth. Soc. Parasitol.





Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2008-02
  1. Identification Of Staphylococcus Aureus Which Causes Mastitis With Mannitol Test And Detection Of Acetoin Product In Dairy Cattke At The Farm Area Of Suka Makmur Dairy Cooperative Grati Pasuruan
  2. The Influence Of Api - Api (avicennia Marina) Leaves Extract Toward Embryo Resorbtion, Body Weight And Lengh Toetal Mice (mus Musculus)
  3. The Effects Of Eimeria Tenella Infection On The Spleen Weight, Size And White Pulp Diameters In The Chickens Primary Infection And Secondary Infection
  4. Detection Of Avian Influenza Virus Subtype H5 On Stray Cats (felis Silvestris Catus) In Bandung City Area
  5. The Effect Of Alkaloid Fraction Of Jarong (achyrantes Aspera Linn) On Viability Culture Myeloma Cell Mice
  6. Antibacterial Activity Of The Ethanol Extract Pluchea Indica Less Leaves Against Escherichia Coli By In Vitro
  7. Identification Of Protein Profile Of Sarcoptes Scabiei On Goats By Sds-page Analysis
  8. Identification Of Excretion-secretion Protein Profile Of The Adult Haemonchus Contortus With Sds-page
  9. Identification Of Aerob Cellulolytic Fungi From Rumen Fluid Waste At Pegirian Slaughter House Surabaya
  10. The Effects Of Supplying Commercial Probiotic On The Quantity And Quality Of Dairy Cow Milk
  11. The Effect Of Given Crude Chlorella To Total Cholesterol Blood Rates On Broiler Chicken
  12. The Effect Sgot And Sgpt Level Of Whiterat ( Rattus Norvegicus ) That Was Given Juice Of Noni ( Morinda Citrifolia ) And High Fatty Diet
  13. Utility Information Technology ( It ) For Head Surface Area Of Spermatozoa Sheep With Biometric Measurement Methode
  14. Influence Of Leaf Extract Of Sirih ( Piper Betle L.) To Growth Staphylococcus Aureus And Escherichia Coli With Disk Diffusion Method
  15. Protein Characterization Of Excretory Secretory Second Stage Larvae Dorman Against Antibody Anti-second Stage Larva Toxocara Canis By Western Blot Techniques

Information FMI@comparison-of-apoptotic-index-and-the-amount-of-brain/VETMED