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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 46 / No. 2 / Published : 2010-10

TOC : 6, and page :120 - 124

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Original Article :

Cytotoxicity effect of curcuma aeruginosa extract on fibroblast with mtt assay method

Author :

  1. Tri Hartini Yuliawati*1
  2. Eka Pramyrtha Hestianah*2
  1. Department of Anatomy and Histology Airlangga University School of Medicine
  2. Department of Anatomy and Histology Airlangga University School of Medicine

Abstract :

The aim of this study was to find cytotoxicity effect of Curcuma aeruginosa extract on fibroblast with MTT assay method. This study used experimental laboratory with post test only control group design. Fibroblast cell lines culture divided into ten groups with four replication in each group. The positif control group was the group contained fibroblas cells in RPMI media. The negatif control group was the group contained media without cells. There were eight treatment groups given Curcuma aeruginosa extract in different concentration ( 1 ppm, 2 ppm, 2.5 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm). After exposure of the extract, the cells were incubated for twenty hours. The MTT reagen was given on the cells and then was incubated for four hours. After that the optic density was read with microplate reader in 630 wavelength. The result showed that the percentage of viable cells was more fifty percent in treatments groups. The Anova analysis showed that there was significantly decrease (p<0,05) of percentage of viable cell between positif control group and treatment groups. The conclusion was the Curcuma aeruginosa extract did not show cytotoxicity effect on fibroblast cells but the percentage of viable cell was decrease significantly in treatment groups.

Keyword :

Curcuma aeruginosa extract, fibroblast, cytotoxicity, MTT assay,


References :

  1. Fawcett, DW, Jensh, RP, (2002). Bloom & Fawcett’s Concise Histology, 2nd edn . New York : Oxford University Press
  2. Fresney, RI, (1987). Culture of Animals Cells (A Manual of Technique), 2nd edn . New York : Liss. Inc.
  3. Grimes, DE, Richard, MG & Hamelink M, (1991). Infectious Diseases . St. Louis : Mosby-Year Book


   


Archive Article

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Volume : 46 / No. : 2 / Pub. : 2010-04
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  4. Differences of tumor necrosis factor alpha (tnf-α) plasma concentration in malaria patients with anemia and without anemia
  5. Differences of epidermal growth factor (egf) concentration between unpreserved and preserved amniotic membrane
  6. Cytotoxicity effect of curcuma aeruginosa extract on fibroblast with mtt assay method
  7. Correlation between blood serotonin level with consciousness level and depression symptoms in moderate brain injury patients
  8. Blood serotonin level with depression situation and neurocognitive as a reflection of neuron condition in six months after moderate brain injury
  9. Association between heart disease in pregnancy with cardiac events
  10. Endoscopic finding of upper gastrointesinal bleeding at dr soetomo hospital, surabaya
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  12. Case report: auditory neuropathy in dr. soetomo hospital