UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 46 / No. 2 / Published : 2010-04

Order : 3, and page :95 - 101

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Original Article :

Diagnostic value of widal slide assay using one phage type local antigen compared with four phage types local antigen in typhoid fever patients in surabaya

Author :

  1. Yetti Hernaningsih*1
  2. Betty Agustina T*2
  3. Aryati*3
  1. Department of Clinical Pathology, Faculty of Medicine, Airlangga University

Abstract :

Widal slide test is one of the serologic tests which are practical, inexpensive and not time consuming. The Widal slide test can be performed in small laboratories, but the sensitivity and specificity depend on the antigen. Salmonella typhi in Indonesia consists of five phage types (D2, A, B1, D6 and E1), however, in Indonesia, it is difficult to differentiate between these phage types. Our objectives is to compare the diagnostic value of Widal slide test with one phage type and four phage types local antigen  in typhoid fever patients. The method in this research is an observational, cross sectional study. Carried out on 78 patients consisting of 39 patients with positive gall culture and 39 non typhoid fever patients with fever and negative gall culture  diagnosed as dengue hemorrhagic fever, malaria, B Hepatitis and Salmonella paratyphi A fever. The results come out that cut off value of Widal slide determined by O or H titer agglutinin was 1/80. The result of Widal slide was considered as positive if O agglutinin titer was more than the cut off value (≥1/160). The sensitivity of Widal slide one phage type was 30.76% (very low) and the specificity was 94.87% (high). Positive Predictive Value was 85.71% (high) and Negative Predictive Value was 57.81% (low), diagnostic efficiency was 62.82% (low). The sensitivity of Widal slide four phage types was 82.05% (high) and the specificity was 82.05 % (high). PPV was 82.05 % (high) and NPV was 82.05 % (high), diagnostic efficiency was 82.05 % (high). Mc. Nemar statistics showed a significant difference in results between Widal slide one phage type and four phage types.  Therefore, we conclude that Widal slide four phage types showed a higher sensitivity and specificity than one phage type. For the future, we suggest Widal slide test to use four or five phage type’s local antigen.

Keyword :

Widal slide local, phage type, Salmonella typhi,


References :

Baron, EJ, Peterson, LR, & Finegold, SM,,(1994) Balley and Scott’s Diagnostic Microbiology, 9th ed. London : The CV Mosby Co

Handojo, I,(2004) Immunoassay untuk Demam Tifoid, in Immunoassay Terapan pada Beberapa Penyakit infeksi Surabaya : Airlangga University Press

WHO,(2003) Background Document: The Diagnosis, Treatment and Prevention of Typhoid fever - : Geneva





Archive Article

Cover Media Content

Volume : 46 / No. : 2 / Pub. : 2010-04
  1. Polymorphism Of Natural-resistance-associated Macrophage Protein 1 (nramp1) D543n Gene And Expression Of Nramp1 On Lung Tuberculosis Patients And Nurses In Surabaya
  2. 45 Kda Fimbria Protein Of Proteus Mirabilis As Hemagglutinin And Adhesion Protein
  3. Diagnostic Value Of Widal Slide Assay Using One Phage Type Local Antigen Compared With Four Phage Types Local Antigen In Typhoid Fever Patients In Surabaya
  4. Differences Of Tumor Necrosis Factor Alpha (tnf-α) Plasma Concentration In Malaria Patients With Anemia And Without Anemia
  5. Differences Of Epidermal Growth Factor (egf) Concentration Between Unpreserved And Preserved Amniotic Membrane
  6. Cytotoxicity Effect Of Curcuma Aeruginosa Extract On Fibroblast With Mtt Assay Method
  7. Correlation Between Blood Serotonin Level With Consciousness Level And Depression Symptoms In Moderate Brain Injury Patients
  8. Blood Serotonin Level With Depression Situation And Neurocognitive As A Reflection Of Neuron Condition In Six Months After Moderate Brain Injury
  9. Association Between Heart Disease In Pregnancy With Cardiac Events
  10. Endoscopic Finding Of Upper Gastrointesinal Bleeding At Dr Soetomo Hospital, Surabaya
  11. Correlation Between Tnf-α, Il-1β, Pge2 And Spla2 Levels With Severity Of Dengue Hemorrhagic Fever
  12. Case Report: Auditory Neuropathy In Dr. Soetomo Hospital

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