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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 44 / No. 1 / Published : 2008-01

TOC : 4, and page :15 - 20

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Original Article :

Difference of interferon gammalevel (release assay) in nurses exposed to mycobacterium tuberculosis and active tuberculous patients

Author :

  1. Endang Retnowati *1
  2. Soedarsono *2
  3. Novi T*3
  1. Department of Clinical Pathology Airlangga University School of Medicine Dr. Soetomo Teaching Hospital, Surabaya
  2. Department of Pulmonary Disease Airlangga University School of Medicine Dr. Soetomo Teaching Hospital, Surabaya
  3. Department of Clinical Pathology Airlangga University School of Medicine Dr. Soetomo Teaching Hospital, Surabaya

Abstract :

Indonesia is a country with a high endemic tuberculosis prevalence. The eradication program is only focused in case finding and treating the active tuberculosis patients. The health care workers are at risk to tuberculosis infection, and there is no method yet to detect and monitor them. In low endemic tuberculosis countries, interferon  (IFN-) can be used as a diagnostic tool to screen the tuberculosis infection in health care workers. The objective of this study was to know the differences of IFN- level by interferon gamma release assay (IGRA) in Mycobacterium tuberculosis-exposed nurse group and active tuberculosis patient's group. A cross sectional, observational analytical study of 13 nurses and 17 active tuberculosis patients, has been conducted from July to December 2006, at Dr. Soetomo General Hospital and Karang Tembok Hospital in Surabaya. There were 3 groups of analysis, first was the nurses group, second was the active tuberculosis patients group I who have been treated for 10.45 weeks and the third was the active tuberculosis patients group II who have been treated for 3.5 weeks. The IFN- assay was done using QuantiFERON-TB Gold In-Tube (Cellestis Ltd, Australia). One mL of whole blood was drawn into 3 tubes, nil control tube, TB antigen tube which contains ESAT-6, CFP-10 and TB7.7 and mitogen tube, then incubated at 37ºC for 16-24 hours. The harvested plasma was then stored at -20ºC until the ELISA was ready to be performed. Results showed that IFN- level in nurses group was 0.12-53.52 IU/mL (mean 10.35 IU/mL, SD 19.49 IU/mL), in the active tuberculosis patients group I was 0.12-26.44 IU/mL (mean 6.08 IU/mL, SD 9.82 IU/mL), and in the active tuberculosis patients group II was 0.14-29.38 IU/mL (mean 8.26 IU/mL, SD 10.91 IU/mL). There were no differences of IFN- level among the 3 groups of analysis, the nurses group, active tuberculosis patients group I and group II by Oneway ANOVA statistical test (p = 0.798). In conclusion, there were no differerences found in IFN- level between the Mycobacterium tuberculosis-exposed nurses group and the active tuberculosis patients groups. Although IFN- level could not be differentiated between these two groups, it is shown that those with high IFN- level in the nurses group should be serially evaluated in order to monitor the possibility of progressiveness to active tuberculosis infection by IFN- measurement, clinical, microbiological and radiological examination.

Keyword :

IFN- release assay, tuberculosis,


References :

  1. Dewan, PK, Grinsdale, J, Kawamura, LM, (2007). Low sensitivity of a whole-blood interferon-γ assay for detection of active tuberculosis . - : Clinical Infectious Disease
  2. Mazurek, MD, Jereb, J, LoBue, P, Iademarco, MF, Metchock, B, Vernon, A, (2005). Guidelines for using the QuantiFERON?-TB gold test for detecting Mycobacterium tuberculosis Infection, United States . - : MMWR Recommendations and Reports


   


Archive Article

Cover Media Content

Volume : 44 / No. : 1 / Pub. : 2008-01
  1. Correlation between dengue virus serotype and humoral immune response in pediatric dengue hemorrhagic fever
  2. Modulation of immunoglobulin g (igg) and cortisol responses in breathing exercise
  3. Prediction of distribution pattern of aedes aegypti as dhf main vector in jember
  4. Difference of interferon gammalevel (release assay) in nurses exposed to mycobacterium tuberculosis and active tuberculous patients
  5. Molecular expression of estrogen receptor alpha (erα) and interleukin 6 (il-6) on acceleration of healing process of long bone shaft fracture by immediate repetitive axial compression tension stabilization (ireact)
  6. Il-4 in peripheral blood mononuclear cells and bronchoalveolar lavage patients with pulmonary tuberculosis before and after treatment with oral anti-tuberculosis drugs
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  10. Review article:challenge of environmental toxicology in reformation era
  11. Bone morphogenetic protein 4 (bmp4) and prostaglandin alpha (pge2α) molecular expression in healing process acceleration of long bone shaft fracture using immediate repetitive axial compression tension stabilization (ireact)
  12. Treatment response of chronic myelogenic leukemia in dr. soetomo hospital
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