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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 43 / No. 4 / Published : 2007-10

TOC : 12, and page :265 - 267

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Original Article :

Effect of acanthus ilicifolius l extract on the reduction of tnf-α expression in wistar strain rattus norvegicus

Author :

  1. I Ketut Sudiana *1
  2. Elyana S Asnar*2
  1. Department of Anatomic Pathology Airlangga University School of Medicine Surabaya
  2. Department of Physiology Airlangga University School of Medicine Surabaya

Abstract :

Vascular lesion is an important factor in the occurrence of atherosclerosis. Vascular lesion can be triggered by collagenase-2 (matrix metalloproteinase-8 or MMP-8). MMP-8 is produced by endothelium resulting from the induction of TNF-a. TNF-a is one of proinflammatory cytokines produced by macrophage. One effort to overcome vascular lesion is the use of the extract of the fruit Acanthus ilicifolius L. However, the effects of this fruit’s extract on the prevention of endothelial lesion were unclear. An experimental study was conducted to find those effects by using post test only control group design, involving 15 experimental animals, comprising 10-week old male Wistar strain Rattus norvegicus. These animals were divided randomly. Group I (5 rats) served as control, group II (5 rats) were induced with Helicobacter pylori LPS in a dose of 0.5 ug/200 gr BW, three times, once for three days, and control group III (5 rats) were induced with H. pylori LPS similar to that of group II but combined with the extract of the fruit Acanthus ilicifolius L in a dose of 70 mg/kg BW in 0.5% CMC per oral/day, for 10 days. Three days after the administration of Acanthus ilicifolius L extract, TNF-a-producing macrophage in the rats' cardiac tissue were examined using immunohistochemistry. The results revealed that TNF-a-producing macrophage in control group and that in LPS-induced group showed significant difference (p < 0.05). The significant difference (p < 0.05) was also found between group receiving LPS and that receiving the combination of LPS and Acanthus ilicifolius L extract. However, control group and the group receiving LPS with Acanthus ilicifolius L extract showed no significant difference (p > 0.05). In conclusion, the fruit Acanthus ilicifolius L has suppressive effect on TNF-a production in macrophage.

Keyword :

atherosclerosis, inflammation, Acanthus ilicifolius L,

References :

  1. Darnell, J, Lodish, H, Baltimore, D, (1990). Molecular Cell Biology, 2nd edn . New York : Scientific American Book
  2. Kuby, (2000). Immunology, 4th edn . New York : WH Freeman and Company
  3. Horiguchi, Y, Uemura, T, Kamata, Y, Kozaki, S, Sakaguchi, G, (1986). Production and caracterization of monoclonal antibodies to clostridium of monoclonal antibodies to clostridium perfringens endotoxin . - : J Infect Immun


Archive Article

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Volume : 43 / No. : 4 / Pub. : 2007-10
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