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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 43 / No. 2 / Published : 2007-04

TOC : 5, and page :87 - 92

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Original Article :

Effect of artificial unilateral ureter total obstructon on serum cystatin-c levels in oryctalagus cuniculus

Author :

  1. Hariyono*1
  2. Soetojo*2
  3. Doddy M Soebadi*3
  4. Endang Retnowati*4
  5. Widodo JP*5
  1. Department of Urology, Gatot Soebroto Army Central Hospital, Jakarta
  2. Department of Urology, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Surabaya
  3. Department of Urology, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Surabaya
  4. Department of Clinical Pathology, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Surabaya
  5. Department of Public Health Administration, Airlangga University School of Public Health, Surabaya

Abstract :

The objective of this study was to determine the effect of artificial unilateral ureter total obstruction on serum Cystatin C levels in Oryctalagus cuniculus. Cystatin-C is a non-glycosylated protein with low molecular-weight (13.36 kD) derived from cystatin super-family of cysteine protease inhibitor. This substance is synthesized constantly by all nucleated cells and not influenced by inflammatory processes, infectious diseases, lean body mass, fever, age, gender or dietary factors. Twenty-two Oryctalagus cuniculus were divided into two groups, 11 were performed artificial unilateral ureter total obstruction and others were performed sham operation as control group. Data of serum cystatin-C were collected on pre operation, day 7 and day 14 from each sample. Serum Cystatin C levels were analyzed with Sandwich ELISA. The data were statistically analyzed with t test. Results showed that the mean serum Cystatin C levels of the male, 3-month old Oryctalagus cuniculus was 0.00671 ± 0.00558 mg/L, day 7 after intervention was 0.01051 ± 0.00536 mg/L and day 14 was 0.019 ± 0.01005 mg/L as examined with Sandwich-ELISA. In conclusion, there was no significant increase of serum Cystatin C on day 7, but by the 14th day a significant increase of serum Cystatin C was observed. There was no significant difference in the increase of serum Cystatin C on day 7 in the intervention group compared to the control group, but there was a significant difference in the increase of serum Cystatin C on the day 14.

Keyword :

serum cystatin-C level, artificial unilateral ureter total obstruction, orictalagus cuniculus,


References :

  1. PT Adi, (2005). Pengaruh obstruksi ureter total unilateral buatan terhadap kejadian apoptosis sel tubulus ginjal dan prolifersai sel tubulus dan fibroblast di interstitial ginjal kontralateral pada Oryctalagus cuniculus. Surabaya : Department of Urology Airlangga University School of Medicine Dr Soetomo Teaching Hospital
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  3. VR Dharnidharka, (2002). Serum Cystatin C is superior to serum creatinin as a marker of kidney function: A meta-analysis . no info : American Journal of Kidney Diseases
  4. B Daryanto, (2005). Pengaruh obstruksi ureter buatan terhadap kejadian apoptosis sel tubulus ginjal pada Oryctalagus cuniculus
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Archive Article

Cover Media Content

Volume : 43 / No. : 2 / Pub. : 2007-04
  1. Detection of mycobacterium leprae dna in blood of the subclinical leprosy
  2. Correlation between mutant p53 protein expression and histopathological grading in astrocytoma patients
  3. Histopathological feature of the tumor of central nervous system at airlangga university school of medicine, dr soetomo hospital, surabaya, in 2001-2003
  4. Exercise diastolic blood pressure as an indicator of endothelial function among subjects with normal and low ankle-brachial index
  5. Effect of artificial unilateral ureter total obstructon on serum cystatin-c levels in oryctalagus cuniculus
  6. The determination antipyrine on plasma by spectrophotometer ultra violet-visible
  7. Lumbar microdiscectomy: technique, advantages and outcomes. surabaya experience on 83 patients
  8. Case report: successful treatment of stevens johnson syndrome-toxic epidermal necrolysis with intravenous immunoglobulin
  9. Review article: hysterosalpingography as a diagnostic test of female infertility
  10. Review article: heart rate variability. a remarkable tool to assess sympathovagal balance
  11. Review article and clinical experience: hypertension in diabetic nephropathy. the five “satan circles”, dha-licoar, the roles of valsartan