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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 44 / No. 1 / Published : 2008-01

TOC : 7, and page :30 - 34

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Original Article :

Effect of oral curcumin and immobilization on the diameter of skeletal muscle fiber in rattus norvegicus

Author :

  1. Ratna Darjanti Haryadi Soebadi*1
  2. I Putu Alit Pawana*2
  1. Department of Physical and Rehabilitative Medicine, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Jl Prof Dr Moestopo 6-8, Surabaya
  2. Department of Physical and Rehabilitative Medicine, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Jl Prof Dr Moestopo 6-8, Surabaya

Abstract :

Immobilization may result in skeletal muscle atrophy, in which the diameter of muscle fiber is decreased. The stimulus response at cellular level presents as the activation of Tumor Necrosis Factor (TNF)- and NF-B (Nuclear Factor-B), which results in the degradation of muscular protein. Curcumin is a yellow pigment substance commonly found in plants belonging to the genus Curcuma longa (kunyit) and Curcuma xanthorrhiza Robx (temulawak). One of the actions of Curcumin is inhibition of the NF-B pathway activation. The objective of this study was to analyze the effect of oral curcumin and immobilization on the diameter of skeletal muscle fiber of Rattus norvegicus. Subjects of the study were male Rattus norvegicus aged 3-4 months, with bodyweight of 150-200 grams. Subjects were divided into three groups, a control group,an immobilization group without curcumin, and an immobilization group with oral curcumin, each of which comprised 11 subjects. Immobilization using a splint was applied to the soleus muscle for 2 weeks. Oral curcumin was given to the immobilization group in a dose of 400 mg/rats (2 g/KgBW) single dose once a week. The diameter of soleus muscle was measured histopathologically with HE staining, under a light microscope using a magnification of 400x. Results showed that the diameter of skeletal muscle fiber in the immobilization group was reduced 42.41% and 26,48% in the immobilization group with curcumin supplementation the reduction was 26.48%, as compared to the control group. There was significant difference (p < 0.05) in the diameter of skeletal muscle fiber between control, immobilization, and immobilization with curcumin supplementation groups. In conclusion, the addition of oral curcumin significantly reduces atrophy of soleus muscle in rats immobilized for 2 weeks.

Keyword :

curcumin, immobilization, skeletal muscle,


References :

  1. Chan, AS & Vallbona, C, (2003). Immobilization, in Handbook of Physical Medicine and Rehabilitation, 2nd edn . Philadelphia : Lippincott William & Wilkins
  2. Farid, M, Reid, MB, Yi-Ping Li et al., (2005). Effects of dietary curcumin or N-acetylcysteine on NF-κB activity and contractile performance in ambulatory and unloaded murine soleus . - : Nutrition & Metabolism


   


Archive Article

Cover Media Content

Volume : 44 / No. : 1 / Pub. : 2008-01
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  6. Il-4 in peripheral blood mononuclear cells and bronchoalveolar lavage patients with pulmonary tuberculosis before and after treatment with oral anti-tuberculosis drugs
  7. Effect of oral curcumin and immobilization on the diameter of skeletal muscle fiber in rattus norvegicus
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