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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 42 / No. 1 / Published : 2006-01

TOC : 6, and page :33 - 39

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Original Article :

Genotyping of extended spectrum Β-lactamase escherichia coli strains from clinical specimens

Author :

  1. Ni Made Mertaniasih*1
  2. Kuntaman*2
  3. Juliette A Severiin*3
  4. Wil Goessens*4
  5. Usman Hadi*5
  6. Nicole Lemmens den Toom*6
  7. Marijam*7
  8. Henri Verbrugh*8
  1. Department of Microbiology, Airlangga University School of Medicine, Dr. Soetomo Teaching Hospital
  2. Department of Microbiology, Airlangga University School of Medicine, Dr. Soetomo Teaching Hospital
  3. Erasmus University Hospital, Rotterdam, The Netherlands
  4. Erasmus University Hospital, Rotterdam, The Netherlands
  5. Department of Internal Medicine, Dr. Soetomo Teaching Hospital
  6. Erasmus University Hospital, Rotterdam, The Netherlands
  7. Department of Microbiology, Airlangga University School of Medicine, Dr. Soetomo Teaching Hospital
  8. Erasmus University Hospital, Rotterdam, The Netherlands

Abstract :

The aim of the present study were to determine the incidence and origin of acquisition of ESBL-producing Escherichia coli strains isolated from Dr. Soetomo university hospital and to delineate the clonal diversity and transmission patterns of these strains by comparing results of genome macro restriction analysis – Pulsed Field Gel Electrophoresis (PFGE). The respective values of the genomic typing methods for epidemiologic typing of Escherichia coli were evaluated in more detail. Clinical specimens as the previous prevalence study of ESBL-E. coli and Klebsiella pneumoniae strains during January 2005 till April 2005 have identified 88 ESBL-E. coli strains of 330 E. coli that identified using standard method-NCCLS and DD synergy test. Banding patterns were PFGE (Certified, Bio Red, CHEF DR II) analyzed using manually read by 3 observers; and strains were defined as having PFGE genotype with ≥ 85 % similarity or no more than 2 band differences. Many strains of 88 members of ESBL-E.coli strains were multi various genotype on PFGE typing; on the other, some strains have the clonal genes, that have identical genotype or identical banding pattern. The clonal spreading of ESBL – Escherichia coli strains had detected event among the wards i.e. Surgery, Pediatry, Internal medicine and Obstetric Gynecology. Appropriate conduct on standard precaution procedures needs reinforcement, possible environmental reservoirs need elimination, and antibiotics policies need reconsideration.

Keyword :

Escherichia coli, genotyping, Extended Spectrum β-Lactamase, PFGE,


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