Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 1 / Published : 2011-01

Order : 6, and page :25 - 29

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Original Article :

Identification of mycobacterium tuberculosis adhesion protein in sputum of tuberculous patients

Author :

  1. Dwi Yuni Nur Hidayati*1
  2. Khusnul Khotimah*2
  3. Uun Yanuhar*3
  1. Laboratory of Microbiology Faculty of Medicine, Brawijaya University
  2. Laboratory of Pharmacology Faculty of Medicine, Brawijaya University
  3. Laboratory of Marine Biotechnology, Faculty of Fisheries and Marine Sciences, Brawijaya University

Abstract :

The pulmonary tuberculosis is an endemic disease in Indonesia. Pulmonary tuberculosis diagnosis at this moment confirmatory using clinical manifestation with detection and identification mycobacteria. Detection and identification of Mycobacteria with culture methode , examination microscopy and moleculer detection with DNA or RNA Mycobacteria. Pendekatan diagnosa tuberkulosis paru secara serologis menggunakan berbagai macam antigen. Diagnosis of phenomenological the pulmonary tuberculosis in a serologic manner using various antigen. The general purpose of this research is to find out the detection of Pili Protein at s-IgA in patient tuberculosis sputum with Immunoblotting examination whereas specific purpose of this research are to find out molecular weight of Pili Protein molecular and also to find out the sensitivity and spesificity of Pili Protein using Immunoblotting method. This research used Mycobacterium tuberculosis Pili as antigen and s-IgA (secretory IgA) as antibody to diagnose pulmonary tuberculosis. At this research the Pili was isolation and determine the molecular weight with electrophoresis SDS PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis). This research also detected that Mycobacterium tuberculosis Pili (MTP) is hemaglutinin molecule and acquire the band of molecular weight of Pili with value 63,63 kDa that response by s-IgA. Therefore, at this research also done the experiment to 96 sample of sputum patient which available that presumable the suffer of pulmonary tuberculosis with dot blot method and Mc Nemar table, the result are sensitivity 71,42% and spesificity with value 63,93% at the cut-off 121,24.

Keyword :

adhesin, hemaglutinin, sIgA, Pili, M. tuberculosis,

References :

Christoper, Alteri J. ,(2007) Mycobacterium tuberculosis produces pili during human infection - : PNAS

Paul W.,(2003) Fundamental Immunology, 5th edition Philadelphia : Lippincott Williams and Wilkins

Raja A,(2004) Immunology of Tuberculosis - : Indian J Med Res

Archive Article

Cover Media Content

Volume : 47 / No. : 1 / Pub. : 2011-01
  1. Evaluation Of Burn Unit Gbpt Performance From 2006-2008. A Retrospective Study
  2. Sex Determinant Of Human Hair With Dna Analysis Method Using Polymerase Chain Reaction (pcr)
  3. Protein Kinase C: After The Combination Of Metformin And Hyperbaric Oxygen Therapy In Type 2 Diabetes Patients
  4. Effectiveness Omeprazole And Lansoprazole In Dyspepsia Patient With Nepean Dyspepsia Index
  5. Decrease Of Cross Shift And Cross Week Blood Cholinesterase Activity In Pesticide Sprayer Of Onion Farmers
  6. Identification Of Mycobacterium Tuberculosis Adhesion Protein In Sputum Of Tuberculous Patients
  7. Correlation Of Histopathological Skin Biopsies With Clinical Diagnosis In Leprosy
  8. Review Article: Reducing Maternal Mortality Rate (mmr) And Infant Mortality Rate (imr): Non Institutional Delivery Or Institutional Delivery?
  9. Maternal Risk Factors Of Premature Delivery In Dr Soetomo Hospital
  10. Citric Acid Reduces The Content Of Pb And Cd Of Kupang Beras (corbula Faba)
  11. Difficulty In Achieving Hemodialysis Adequacy In Hemodialysis Unit, Dr. Soetomo Hospital, Surabaya, Indonesia
  12. Acute Toxicity Test Of Bark And Stem Ethanol Extract Of Sopang (caesalpinia Sappan Linn) By Brine Shrimp Lethality Test
  13. Penile Length Of Newborn Infants In Dr. Soetomo Hospital Surabaya. A Preliminary Study