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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 51 / No. 2 / Published : 2015-04

TOC : 13, and page :142 - 148

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Original Article :

Immunochromatography method for rotavirus detection provides good accuracy in diagnosis of rotavirus diarrhea in children

Author :

  1. Sherly Yuniarchan*1
  2. Andy Darma*2
  3. Alpha Fardah Athiyyah*3
  4. IGM Reza Gunadi Ranuh*4
  5. Subijanto Marto Sudarmo*5
  6. Boediono*6
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran
  5. Dosen Fakultas Kedokteran
  6. Dosen Fakultas Kedokteran

Abstract :

Rotavirus infections are a major cause of diarrhea in children under 5 years in both the developed and developing countries. Goldstandard diagnostic method for rotavirus diarrhea is RT-PCR but it is expensive and longtime. Immunochromatography is a newdiagnostic method that is fast, easy and cheap but the variation in diagnostic accuracy is still very wide in various countries. Theobjective of this study was to evaluate the accuracy of the immunochromatography method as compared to RT-PCR method in thediagnosis of rotavirus diarrhea in children. Cross-sectional study was conducted in children aged 1-60 months with acute diarrheahospitalized at gastroenterology ward Dr. Soetomo Hospital on January until June 2014. Patients, who had received rotavirusimmunization 1 month before the test, were excluded from this study. Immunochromatography method was using BD Rota/AdenoExamine™ stick (Beckton Dickinson Japan Co., Ltd.). Identification of rotavirus by RT-PCR was performed at the Institute ofTropical Disease (ITD), Airlangga University. Sensitivity, and specificity, positive predictive value (PPV), negative predictive value(NPV) and likelihood ratio (LH) were calculated for the immunochromatography method. A total of 80 children were included.Thesensitivity, specificity, PPV, NPV and LH of immunochromatography respectively were 77.6%, 63.6%, 84.9%, 51.85% and 2.13. Inconclusion, immunochromatography method for rotavirus detection provides good accuracy in diagnosis of rotavirus diarrhea inchildren. (FMI 2015;51:142-148)

Keyword :

immunochromatography, rotavirus diarrhea, diagnostic accuracy, children,


References :

  1. Lundgren O, Svensson L , (2001). Pathogenesis of rotavirus diarrhea. 3, 1145-1156 : Microbes Infect
  2. Manjula G , (2013). Comparison of immunochromatography with RT-PCR for detection of rotavirus in fecal samples. 2, 479-481 : International Journal of Scientific Research


   


Archive Article

Cover Media Content

Volume : 51 / No. : 2 / Pub. : 2015-04
  1. Implementation of pharmaceutical care in resolving drug problems in type 2 diabetes mellitus
  2. Comparison of the effect of statin types on the reduction of lipid profile and hs-crp inflammatory marker in diabetics with dyslipidemia
  3. Ciprofloxacin is effective in benign prostatic hyperplasia (bph) patients with urethral catheters
  4. Procalcitonin as biomarker of antibiotic effectivity in communityacquired pneumonia
  5. Effect of fursultiamine supplementation on left ventricular ejection fraction (lvef) in grade iii – iv heart failure patients
  6. Diagnostic value of st2 in patients with acute myocardial infarction
  7. Molecular analysis of rpob gene of mycobacterium tuberculosis in patients with suspected pulmonary tuberculosis in surabaya
  8. Liver function tests of patients with dengue fever, dengue hemorrhagic fever and dengue shock syndrome in tropic and infectious disease ward in the department of internal medicine, dr. soetomo hospital, surabaya
  9. Case report: dengue fever with perimyocarditis
  10. Correlation between heat shock protein (hsp) 70 level and left ventricular dysfunction and its effect on cardiovascular and rehospitalization incidence in patients with acute coronary syndrome
  11. Effect of tomato (lycopersicum commune) juice on vaginal wall elasticity in menopausal rats
  12. Acetosal resistance in coronary artery disease between and within type 2 diabetes mellitus patients
  13. Immunochromatography method for rotavirus detection provides good accuracy in diagnosis of rotavirus diarrhea in children