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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

TOC : 1, and page :68 - 73

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Original Article :

Immunogenity of 32.2 kda hemagglutinin bm protein of escherichia coli pili isolated from infertile males' semen

Author :

  1. Sukarjati*1
  2. Doddy M Soebadi*2
  3. Aucky Hinting*3
  4. Sudjarwo*4
  1. Department of Biology, PGRI Adi Buana University, Surabaya
  2. Department of Urology, Airlangga University Faculty of Medicine, Surabaya
  3. Department of Medical Biology, Airlangga University Faculty of Medicine, Surabaya
  4. Department of Pharmacochemistry, Airlangga University Faculty of Pharmacy, Surabaya

Abstract :

Male genital tract infection is one cause of infertility. Escherichia coli (E. coli) is a major cause of prostatitis and epididymitis that can degrade the quality of spermatozoa. One factor E. coli can reduce sperm quality due to the ability of E. coli attached to the sperm plasma membrane using pili. Have successfully isolated the protein hemagglutinin pili of E. coli isolates were infertile men with BM cement 32.2 kDa. The purpose of this study was to determine the protein hemagglutinin pili immunogenitas E. BM coli isolates 32.2 kDa cement infertile men. Immunization performed on male rabbits using protein hemagglutinin pili of E. coli BM 32.2 kDa as antigens. Antibody titers formed were measured using indirect methods and the results were read using elisa reader. Antigen and antibody reactivity are formed in the test by the method of dot blot and western blot. The results of this research is through indirect elisa test, the protein hemagglutinin pili of E. BM coli isolates 32.2 kDa cement infertile men can stimulate the formation of antibodies. The results of dot blot test and western blot proved that the antibodies react specifically with antigen. The conclusion of this study is the protein hemagglutinin pili of E. coli BM 32.2 kDa is immunogenic and potentially as a candidate vaccine ingredient in preventing male genital tract infections.

Keyword :

protein hemagglutinin pili of E. coli BM 32.2 kDa, immunogenity, indirect elisa, western blot, dot blot,


References :

  1. Aronson,M.O., Medalia O., Schori, L., Mirelman, D., Sharon, N., Ofek, I., , (1979). Prevention of colonization of the urinary tract of mice with E. coli by blocking of bacterial adherence with methyl-alpha-mannopyranoside. - : J.Infect.Dis.
  2. Connell,H., Agace,W., Klemm, P., Schembri, M., Marild,S., Svanborg,C., (1996). Type 1 fimbrial expression enhances E. coli virulence for the urinary tract. - : Proc.Natl. Acad.Sci
  3. Cummins JM., (1995). Tests of Sperm Function. Cambridge : Cambridge University Press
  4. Diemer T., Weidner W., Michelmann HW., Schiver HG., Rovan E., and Mayer F., (1996). Influence of E. coli on motility parameters of human spermatozoa in vitro. - : Int. J. of Andrology
  5. Henahan S., (1997). E. coli Vaccine. - : Science Update


   


Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
  1. Immunogenity of 32.2 kda hemagglutinin bm protein of escherichia coli pili isolated from infertile males' semen
  2. Role of ehsp 72 on the increase of il-6 and nk cells in participants of majelis dzikir
  3. Effect of 2-methoxyethanol on sperm count, leucocyte and epididymal epithelial cell structure in rats (rattus norvegicus)
  4. Reversin increase the plasticity of bone marrow-derived mesenchymal stem cell for generation of cardiomyocyte in vitro
  5. Detection of cholera toxin-producing vibrio cholerae aquatic isolates in kalimas river-surabaya
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  12. Serological survey on avian influenza subtype h5n1 viral antibody in healthy blood donor in surabaya
  13. Effect of topical hyaluronate and freeze-dried amnion membrane administration on ck 16 protein expression and the number of epithelial layers in superficial wound of male wistar strain rats