UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 41 / No. 1 / Published : 2005-01

Order : 8, and page :43 - 48

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Original Article :

In vitro drug sensitivity of trypanosoma evansi bangkalan isolates

Author :

  1. Mochamad Lazuardi*1
  1. Department of Veterinary Pharmacy Airlangga University School of Veterinary Medicine Surabaya

Abstract :

Only a limited number of drugs is available for Trypanosoma infection treatment. In Indonesia there are two chemoterapheutics (isometamidium and diminazene) used for treatment. The isometamidium does not act as a trypanocide of Indonesia isolates. However, the clinical action of diminazene to Trypanosoma has not been reported yet. The objective of this research was to obtain trypanocide concentration of Trypanosoma evansi Indonesia isolates by in vitro test. Drug sensitivities of Trypanosoma evansi Bangkalan isolates modified using Minimum Eagle Medium and HEPES medium were measured for diminazene aceturate and suramin using in vitro incorporation test. In control group, trypanosome inoculate was added as a placebo substance in microtiter plate containing trypanosomes. Bloodstream forms were isolated from Wistar strain Rattus norvegicus, incubated at room temperature in serial drug dilutions in a microtiter plate by adding 5 % of CO2. After 24 hours, the trypanosomes were counted by inverted microscope to determine trypanocide action. The two subjects research were different trypanocide action (p<0.05). The minimum concentration with trypanocide action of diminazene aceturate was found to be 8.480 μg.ml(-1).The minimum concentration with trypanocide action of suramin was 6.600 μg.ml(-1).

Keyword :

diminazene aceturate, suramin, Trypanosoma,


References :

Lazuardi M,(1998) The trypanocide effect of suramin against to Trypanosoma evansi - : The Indonesian Journal of Parasitology

Van Nieuwenhove S,(1992) Advances in sleeping sickness theraphy - : Ann. Soc. Belge Med. Trop





Archive Article

Cover Media Content

Volume : 41 / No. : 1 / Pub. : 2005-01
  1. Editorial Vol 41 No 1 2005
  2. Opinion: Research In The Development Of Medical Science And Technology
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  4. The Effects Of Unilateral Testicular Torsion Upon Immunity Modulation And Apoptosis Of Germinal Cells In The Contralateral Testis. An Experimental Study In Rats
  5. The Effect Of 5α Reductase Inhibitor And Estrogen In Prostate Proliferation. An Experimental Study In Rats
  6. Quality Of Refilled Drinking Water In Surabaya City
  7. Adequate Help For Patients With Cervical Cancer? The Referral System In Indonesia. A Descriptive Comparison Study In Four Provinces
  8. In Vitro Drug Sensitivity Of Trypanosoma Evansi Bangkalan Isolates
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  10. Serum Osmolal Gap In Healthy Persons. Comparison Of Eleven Formulas For Calculating Osmolality
  11. Traumatic Optic Neuropathy In The Division Of Neuro-ophthalmology, Department Of Ophthalmology, Dr Soetomo Teaching Hospital, Surabaya
  12. The Potential Role Of α-lipoic Acid In The Management Of Diabetes Mellitus. Possible Molecular Mechanism
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