Folia Medica Indonesiana
ISSN 0303-7932
Vol. 46 / No. 3 / Published : 2010-07
Order : 4, and page :179 - 185
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Original Article :
Mapping pattern of core dna loci in forensic identification sample examination using polymerase chain reaction (pcr) in loci csf1po, tho1 and tpox
Author :
- Ahmad Yudianto*1
- Soekry Erfan Kusuma*2
- Department of Forensic and Medicolegal Faculty of Medicine, Airlangga University, Human Genetic Study Group Team TDC Airlangga University
- Department of Forensic and Medicolegal Faculty of Medicine, Airlangga University, Human Genetic Study Group Team TDC Airlangga University
Abstract :
The identification of victims and suspects in cases of crime such as murder, rape with a murder which is increasingly also increase the quantity and quality, increasingly confirmed the existence of forensic medicine. So far the molecular identification in forensic cases with failure of conventional identification has not been much in the know. This study aims to explain the molecular forensic methods of identification in case of failure of identification by conventional methods. The environmental affects DNA content. As is known environmental factors such as humidity and temperature environment is affecting the condition of DNA that are used as materials in the field of forensic DNA identification, as in any other field of DNA examination. The environmental factors affecting DNA degraded or degraded DNA. This degradation can be fast or slow, it depends on factors that influence and timing of exposed. This study showed that all STR loci tested both on samples of blood spots and patches of sweat from day 1, day 10 and day of the 20 shows can still be detected through visualization electrophoresis polyacrilamid agarose gel. The success on these three STR loci, which in this study found the sequence from small to big success is CSF1PO, TPOX and THO1. It was found that the locus TPOX THO1 or equally likely in the success of the examination compared with the STR loci CSF1PO. This is consistent with the calculation of the ratio of GC content and TPOX THO1 have the same relative value that is 0.48, compared with CSF1PO namely 0.33.
Keyword :
STR loci , TPOX THO1, blood spots, patches of sweat,
References :
Atmaja DS,(2005) Peranan Sidik Jari DNA pada Bidang Kedokteran Forensic. Materi Workshop DNA Fingerprinting Yogyakarta : Universitas Gadjah Mada
Butler JM, Shen Y, McCord BR,(2004) The Development of Reduced Size STR Amplicons as Tools for Analysis of Degraded DNA - : National Institute of Standards and Technology
Muladno,(2002) Seputar Teknologi Rekayasa Genetika. 1st ed Bogor : Pustaka Wirausaha Muda
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