UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 46 / No. 3 / Published : 2010-07

Order : 4, and page :179 - 185

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Original Article :

Mapping pattern of core dna loci in forensic identification sample examination using polymerase chain reaction (pcr) in loci csf1po, tho1 and tpox

Author :

  1. Ahmad Yudianto*1
  2. Soekry Erfan Kusuma*2
  1. Department of Forensic and Medicolegal Faculty of Medicine, Airlangga University, Human Genetic Study Group Team TDC Airlangga University
  2. Department of Forensic and Medicolegal Faculty of Medicine, Airlangga University, Human Genetic Study Group Team TDC Airlangga University

Abstract :

The identification of victims and suspects in cases of crime such as murder, rape with a murder which is increasingly also increase the quantity and quality, increasingly confirmed the existence of forensic medicine. So far the molecular identification in forensic cases with failure of conventional identification has not been much in the know. This study aims to explain the molecular forensic methods of identification in case of failure of identification by conventional methods. The environmental affects DNA content. As is known environmental factors such as humidity and temperature environment is affecting the condition of DNA that are used as materials in the field of forensic DNA identification, as in any other field of DNA examination. The environmental factors affecting DNA degraded or degraded DNA. This degradation can be fast or slow, it depends on factors that influence and timing of exposed. This study showed that all STR loci tested both on samples of blood spots and patches of sweat from day 1, day 10 and day of the 20 shows can still be detected through visualization electrophoresis polyacrilamid agarose gel. The success on these three STR loci, which in this study found the sequence from small to big success is CSF1PO, TPOX and THO1. It was found that the locus TPOX THO1 or equally likely in the success of the examination compared with the STR loci CSF1PO. This is consistent with the calculation of the ratio of GC content and TPOX THO1 have the same relative value that is 0.48, compared with CSF1PO namely 0.33.

Keyword :

STR loci , TPOX THO1, blood spots, patches of sweat,


References :

Atmaja DS,(2005) Peranan Sidik Jari DNA pada Bidang Kedokteran Forensic. Materi Workshop DNA Fingerprinting Yogyakarta : Universitas Gadjah Mada

Butler JM, Shen Y, McCord BR,(2004) The Development of Reduced Size STR Amplicons as Tools for Analysis of Degraded DNA - : National Institute of Standards and Technology

Muladno,(2002) Seputar Teknologi Rekayasa Genetika. 1st ed Bogor : Pustaka Wirausaha Muda





Archive Article

Cover Media Content

Volume : 46 / No. : 3 / Pub. : 2010-07
  1. Socioeconomic And Genetic Factors Influencing The Strength, Weight, Length And Width Measurements Of Children
  2. Glomerular Filtration Rate In Liver Cirrhosis
  3. Antimicrobial Activity Of Mahkota Dewa [phaleria Macrocarpa (scheff). Boerl.] Leaf Extract Against Pseudomonas Aeruginosa By Agar Dilution And Scanning Electron Microscopy
  4. Mapping Pattern Of Core Dna Loci In Forensic Identification Sample Examination Using Polymerase Chain Reaction (pcr) In Loci Csf1po, Tho1 And Tpox
  5. Spatial Analysis Of Dengue Fever Regarding Its Determinant Factors In Bandar Lampung
  6. Inhibition Of The Mitogen Activated Protein Kinase (mapk) In The Inflammatory Pain Like State Using Sb 203580 And Pd 98059 In Mice
  7. Cloning Of Melanoma Antigen-1 (mage-1) Gene From Fine Needle Aspiration Biopsy Of Hepatic Tissue Of Hepatocelluler Carcinoma Patients
  8. Detection Of Urinary Incontinence Affecting Post-menopausal Age Using Ii Q-7 And Udi-6 Questionnaire At Immanuel Teaching Hospital Bandung
  9. Electron Microscopic Scanning Profile Of Balb/c Mice Intestinal Villi After Per Oral Lps (lipopolysaccharide) During Probiotic Induction
  10. Extensive Intensity Exercise Most Effectively Increases Anaerobic Threshold
  11. Pegagan (centella Asiatica) Extract Increases Vaginal Wall Thickness In Menopausal Rats
  12. Review Article: Interval Exercise With 1:1 Work/rest Ratio Decreases The Risk Factors Of Type-2 Diabetes Mellitus And Coronary Heart Disease
  13. Expression Of Topoisomerase Iiα And Cyclin D1 Proteins In Various Degrees Of Breast Ductal Carcinoma