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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 51 / No. 2 / Published : 2015-04

TOC : 7, and page :110 - 113

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Original Article :

Molecular analysis of rpob gene of mycobacterium tuberculosis in patients with suspected pulmonary tuberculosis in surabaya

Author :

  1. Nurul Wiqoyah*1
  2. Ni Made Mertaniasih*2
  3. Manik Retno W*3
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

Tuberculosis caused by Mycobacterium tuberculosis is still a public health problem in Indonesia. Important initial step in an effort tocontrol TB and MDR-TB is TB case early finding, so early and accurate diagnosis is very important to deal with TB cases quicklyand accurately as well. Rapid molecular diagnostic methods based on molecular specific genes of Mycobacterium tuberculosisrequire the data from the genome sequence of the bacterium. RpoB gene is a gene that can be used as a bacterial diagnostic toolwhich is responsible for anti-TB drug rifampicin resistance that showed mutations in certain areas, and varied geography. So thesequences of the ropB gene of Mycobacterium tuberculosis need to be known. Research methods include the extraction andamplification of DNA regions rpoB gene of Mycobacterium tuberculosis clinical isolates from suspected of pulmonary TB patients,followed by sequencing. Ten isolates of Mycobacterium tuberculosis from sputum of patients suspected of pulmonary TB wereobtained. Amplification of a region of the rpoB gene of Mycobactrium tuberculosis resulst in a 411 bp and sequences ofMycobacterium tuberculosis rpoB gene region showed the same sequence as the nucleotide sequence of Mycobacterium tuberculosisH37Rv. These studies concluded that the region of 411 bp rpoB gene amplification product has a 100% DNA sequence homologywith Mycobacterium tuberculosis H37Rv.(FMI 2015;51:110-113)

Keyword :

rpoB gene, rifampicin, Mycobacterium tuberculosis, sequencing,

References :

  1. Adékambi T, Drancourt M, Raoult D , (2009). The rpoB gene as a tool for clinical microbiologists. 17, 37-45 : Trends Microbiol
  2. Hauck Y, Fabre M, Vergnaud G, Soler C, Pourcel C , (2009). Comparison of two commercial assays for the characterization of rpoB mutations in Mycobacterium tuberculosis and description of new mutations conferring weak resistance to rifampicin. 64, 259-262 : J Antimicrob Chemother


Archive Article

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Volume : 51 / No. : 2 / Pub. : 2015-04
  1. Implementation of pharmaceutical care in resolving drug problems in type 2 diabetes mellitus
  2. Comparison of the effect of statin types on the reduction of lipid profile and hs-crp inflammatory marker in diabetics with dyslipidemia
  3. Ciprofloxacin is effective in benign prostatic hyperplasia (bph) patients with urethral catheters
  4. Procalcitonin as biomarker of antibiotic effectivity in communityacquired pneumonia
  5. Effect of fursultiamine supplementation on left ventricular ejection fraction (lvef) in grade iii – iv heart failure patients
  6. Diagnostic value of st2 in patients with acute myocardial infarction
  7. Molecular analysis of rpob gene of mycobacterium tuberculosis in patients with suspected pulmonary tuberculosis in surabaya
  8. Liver function tests of patients with dengue fever, dengue hemorrhagic fever and dengue shock syndrome in tropic and infectious disease ward in the department of internal medicine, dr. soetomo hospital, surabaya
  9. Case report: dengue fever with perimyocarditis
  10. Correlation between heat shock protein (hsp) 70 level and left ventricular dysfunction and its effect on cardiovascular and rehospitalization incidence in patients with acute coronary syndrome
  11. Effect of tomato (lycopersicum commune) juice on vaginal wall elasticity in menopausal rats
  12. Acetosal resistance in coronary artery disease between and within type 2 diabetes mellitus patients
  13. Immunochromatography method for rotavirus detection provides good accuracy in diagnosis of rotavirus diarrhea in children