Catalog :




Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 4 / Published : 2011-10

TOC : 6, and page :224 - 229

Related with : Scholar   Yahoo!   Bing

Original Article :

Nucleic acid amplification of the rpob region of mycobacterium tuberculosis in pulmonary tuberculosis diagnosis

Author :

  1. Yudita Wulandari*1
  2. Nurul Wiqoyah*2
  3. Ni Made Mertaniasih*3
  1. Department of Medical Microbiology, Faculty of Medicine, Airlangga University
  2. Department of Medical Microbiology, Faculty of Medicine, Airlangga University
  3. Department of Medical Microbiology, Faculty of Medicine, Airlangga University

Abstract :

Tuberculosis (TB) is one of the major public health concerns worldwide. The detection of the pathogen Mycobacterium tuberculosis complex (MTBC) as early as possible has a great impact on the effective control of the spread of the disease. It is difficult to diagnose Mycobacterium tuberculosis infection due to a lack of rapid, sensitive and specific test. Newer methods, which are easy and reliable, are required to diagnose TB. This research aim is to evaluate the accuracy polymerase chain reaction (PCR) technique, using primers the rpoB gene region compare to culture method in Lowenstein-Jensen medium as a gold standard for the detection of Mycobacterium tuberculosis in the sputum samples. Sputum samples from TB suspected patients are examined by culture and PCR, using rpoB target gene. Specimens are digested and decontaminated by the modified Petroff method (WHO). Approximately from 1.0 ml of resuspended sediment, each 100 ul is used to inoculate Lowenstein-Jensen slants in duplo and 100 ul resuspended sediment is processes for PCR. Mycobacterium tuberculosis is identified using a specific pair of primers designed to amplify 541 bp sequences of rpoB gene. Conclusion: PCR have the high accuracy, sensitivity 100% and specificity 100% for pulmonary TB diagnosis. The performance of a rpoB Mycobacterium tuberculosis PCR assay have value in the rapid diagnosis of pulmonary tuberculosis.

Keyword :

rpoB gene, Mycobacterium tuberculosis, diagnosis,


References :

  1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhi K, (1990). Current protocols in moleculer biology. Vol. 2. New York : Greene Publishing Associates and Wiley-Interscience, John Wiley & Sons
  2. Boddinghaus I, Rogall T, Flohr T, Blocker H, Bottger EC, (1990). Detection and identification of Mycobacteria by amplification of rRNA. - : J. Clin. Microbiol
  3. Damle AS, Kaundiya DV, (1986). Comparison of three methods of decontamination of sputum for mycobacterial culture. - : Indian Journal of Tuberculosis
  4. Donnabella V, F. Martiniuk, D. Kinney, M. Bacerdo, S. Bonk, B. Hanna, W. N. Rom, (1994). Isolation of the gene for the beta subunit of RNA polymerase from rifampicin-resistant Mycobacterium tuberculosis and identification of new mutations. - : Am. J. Respir. Cell Mol. Biol.
  5. Filho LDA, Celemann WMR, Barreto CEN, Kritski AL, Fonseca Ls, (2002). Sensitivity of Amplicor MTB on direct detection of Mycobacterium tuberculosis in smear-negative specimens from outpatients in Rio de Janeiro. - : Brazilian J Microbiol


   


Archive Article

Cover Media Content

Volume : 47 / No. : 4 / Pub. : 2011-10
  1. Antibiotic resistance control program (arcp) improving antibiotic use in pediatric hematology and oncology patients at dr soetomo hospital in 2006 and 2008
  2. Sensitivity and specificity of clinical features of influenza like illness and laboratorical findings for diagnosed influenza a (h1n1) in children
  3. Risk factors for birth asphyxia
  4. Liver involvement in children with dengue infection
  5. Mechanical ventilation vs nasal cpap duration and the risk of late onset sepsis in premature babies with respiratory distress syndrome
  6. Nucleic acid amplification of the rpob region of mycobacterium tuberculosis in pulmonary tuberculosis diagnosis
  7. Effect of oral-estrogen conjugate on b-lymphocyte proliferation in the peyer’s patches of adult female balb/c mice ileum
  8. The effect of emotional quality management on stress, and optimism in women with breast cancer
  9. Quality and efficiency improvement of professional medical doctor education in public health field using objective structured public health examination (osphe) approach at faculty of medicine airlangga university
  10. Review article: role of ve-cadherin in vascular permeability regulation
  11. Depth of human hypophysial fossa due to aging process: a morphologic research