Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 45 / No. 4 / Published : 2009-10

Order : 6, and page :278 - 284

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Original Article :

Pcr inhibitor contaminant detection from extraction products of resin chelating dna in forensic dna identification

Author :

  1. Agung Sosiawan *1
  2. Soekry Erfan Kusuma*2
  1. Department of Oral Biology, Faculty of Dentistry, Airlangga University
  2. Department of Forensic Medicine and Medicolegal, Faculty of Medicine, Airlangga University

Abstract :

One crucial thing to note in forensic DNA identification is the process of DNA extraction. This research is expected to give an answer will still need information about whether the decalcification process using chelating agent EDTA ingredient in the bone used as ingredients in forensic DNA examination, in the process of DNA extraction using chelating resin, which is also a chelating agent material, where with the material chelating agent in it is feared would complicate the process of DNA amplification using PCR. DNA concentration obtained from the extraction of DNA by chelating resin method with decalcification of bone have different results with the DNA content of the extracted DNA by the same method without decalcification, the DNA content of the extracted DNA by decalcification has a tendency more than the content of the extracted DNA DNA with decalcification. Nuclear DNA and mitochondrial DNA of bone, each on the examination of Short Tandem Repeat (STR) loci VWA and examination of mitochondrial DNA Hypervariable region 1 (HV1) 143 bp amplification product of the method of extraction with chelating resin with or without decalcification without accompanied by precipitation with alcohol, have a lower success rate compared with the results of DNA extraction with or without decalcification alokohol accompanied by precipitation, using the same method in the examination. EDTA and chelating resin material which has the properties of chelating agent can be inhibitors on the reaction process Polymerase Chain Reaction (PCR).

Keyword :

PCR, DNA, forensic, resin, chelating,

References :

  1. Kolman, CJ, and Tuross, N, (2000). Ancient DNA analysis of human populations . - : Am. J. Phys. Anthropol
  2. Smith BC, Fisher DL,WeednVW,Warnock GR, Holland MM, (1993). A systematic approach to the sampling of dental DNA . - : Journal of International Forensic Science

Archive Article

Cover Media Content

Volume : 45 / No. : 4 / Pub. : 2009-10
  1. Utilization of extract phaleria (phaleria macrocarpa) as a precaution against carbon tetrachloride induced hepatotoxicity in mice
  2. Effect of transventricular cerebrospinal fluid in patient with severe brain damage with thin acute subdural bleeding. a comparative study with decompression trepanation action
  3. Comparative effectiveness between chlorhexidine gluconate 0.2% and povidone-iodine 1% as an antiseptic to decrease density level of intraoral germs in maxillofacial fracture surgery
  4. Cyclin d1 expression relationship with clinical stage, grading gland metastasis of tumor cells and squamous cell in cervical carcinoma
  5. Identification of fungtional cluster of extract parasite duku (loranthaceae deandropthoe species) with ft-ir spectrometer
  6. Pcr inhibitor contaminant detection from extraction products of resin chelating dna in forensic dna identification
  7. Events and risk factors of developmental disorders in low birth weight babies
  8. Correlation of c-erbb2 expression in undifferentiated nasopharyngeal carcinoma and tumor size (t-stage), regional lymphnodes status (n-stage), and radiation response
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  10. Parasitological examination of imported and indigenous malaria in tegalombo community health center, pacitan disctrict, east jawa province
  11. Review article: how to manage endometrioma by laparoscopy? ablation or excision?
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