Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 50 / No. 2 / Published : 2014-04

Order : 4, and page :81 - 85

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Original Article :

Profile of gyra and gyrb gene mutation in clinical isolate of ciprofloxacin resistant pseudomonas aeruginosa

Author :

  1. Erwin Firman S*1
  2. Didik Hasmono*2
  3. Kuntaman*3
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Dosen Fakultas Kedokteran

Abstract :

Nosocomial infections can increase morbidity, mortality, and also the cost of treatment because hospital care becomes longer. Oneof the most bacteria causing nosocomial infections is Pseudomonas aeruginosa. Ciprofloxacin is the most powerfulantipseudomonal, which has 4-8 times more active than levofloxacin, against Pseudomonas aeruginosa. Ciprofloxacin antibioticresistance can be caused by mutations in DNA gyrase and topoisomerase IV. DNA gyrase mutations in bacteria Pseudomonasaeruginosa can occur in genes gyrA and gyrB. This study aims to see gyrA and gyrB gene mutations in clinical isolates ofPseudomonas aeruginosa were resistant to ciprofloxacin by using PCR QiaQuick column (QIAGEN, Valecia, CA) and the ABI Prism310 Genetic Analyzer. All 5 Pseudomonas aeruginosa isolates of showed phenotypes that were resistant to ciprofloxacin and gyrAgene mutation in the amino acid change at Thr83 to be Ile at 100%. This suggests that mutations in the gyrA gene affects theoccurrence of bacterial resistance of Pseudomonas aeruginosa to ciprofloxacin. (FMI 2014;50:81-85)

Keyword :

Pseudomonas aeruginosa, gyrA gene, gyrB gene, mutation, ciprofloxacin,

References :

Drlica K, Malik M, Kerns RJ, Zhao X,(2008) Quinolone-Mediated Bacterial Death - : Antimicrob Agents Chemother

Lee JK, Lee YS, Park YK, Kim BS,(2005) Alterations in the GyrA and GyrB subunits of topoisomerase II and the ParC and ParE subunits of topoisomerase IV in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa - : Int J Antimicrob Agents

Archive Article

Cover Media Content

Volume : 50 / No. : 2 / Pub. : 2014-04
  1. The Effect Of 3000 Mg Intravenous Vitamin C On Serum Nitric Oxide Level Of Severe Burnt Injury Patients
  2. Correlation Between Plasma Levels Of Interferon And Viral Load In Patients With Hiv Stage 1
  3. Correlation Between Serum Level Of Heart Type Fatty Acid Binding Protein (h-fabp) And N-terminal Pro-brain Natriuretic Peptide (nt-probnp) On Acute Coronary Syndrome Patient
  4. Profile Of Gyra And Gyrb Gene Mutation In Clinical Isolate Of Ciprofloxacin Resistant Pseudomonas Aeruginosa
  5. Effect Of Cardiac Rehabilitation Programs On Functional Capacity Of Post Coronary Revascularization Patient Using Six Minute Walk-test
  6. Correlation Between Tgf-1 Plasma Level And Cd4+ T Lymphocyte Count In Stage I Hiv-infected Patients
  7. The Effect Of 3000 Mg Vitamin C Infusion On Histamine Level Of Severely Burnt Patients
  8. Comparison Of Fugl-meyer Score Between Patients With Acute Thrombotic Infarc Stroke Treated With Standard Medication And Fluoxetine
  9. Antibiotics Quantity-based Escherichia Coli Resistance Pattern In Patients At Surgical And Psychiatric Wards, Dr Soetomo Hospital, Surabaya
  10. Age And Resting Heart Rate Are Discriminators To Predict Endurance Without Physical Test
  11. Case Report: Trunk And Reticular Varicose On A 9-year-old Child
  12. Comparison Of The Apgar Score Of Spontaneous Vaginal Birth And Assisted Birth In Dr. Soetomo Hospital Surabaya
  13. Five Years Retrospective Study Of Burns In Dr Soetomo General Hospital Surabaya