Catalog :

Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 49 / No. 2 / Published : 2013-04

TOC : 8, and page :109 - 111

Related with : Scholar   Yahoo!   Bing

Original Article :

Quantitation of microorganisms in lower respiratory tract secretions

Author :

  1. Manik R. Wahyunitisari*1
  2. Kuntaman*2
  3. Ni Made Mertaniasih*3
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

The usefulness of lower respiratory tract secretions culture in guiding microbiological diagnosis of pneumonia is controversial. Dueto the inevitable oropharyngeal bacterial contamination that occurs in the collection of all respiratory secretion samples,quantitative culture techniques are always needed to differentiate oropharyngeal contaminants present at low concentration fromhigher-concentration infecting organisms. A number of 783 patients from Dr. Soetomo hospital Surabaya on 2012 with pneumoniawere studied. Before administering antibiotic therapy, lower respiratory tract secretion was collected and processed according tostandard protocols. Quantitation of sputum from patients with pneumonia revealed that probable pathogens were present inpopulation of ≥ 107 organisms/ml. The threshold for positive culture defining pneumonia was 104 cfu/ml for bronchoalveolar lavage(BAL) and 105 cfu/ml for endotracheal aspirate (EA). There were 384 gram-positive cocci, and 307 gram-negative bacilli.Quantitation of microorganisms in lower respiratory tract secretion yielded a causative organism in 691 (88%) of the 783 samples.In conclusion, lower respiratory tract secretions with quantitative culture can be helpful for the diagnosis of bacterial pneumonia.(FMI 2013;49:109-111)

Keyword :

quantitative culture techniques, pneumonia, sputum, BAL, EA,

References :

  1. American Thoracic Society, (1996). Hospital-acquired pneumonia in adults: diagnosis, assessment of severity, initial antimicrobial therapy, and preventive strategies. - : Am J Respir Crit Care Med
  2. Canadian Critical Care Trials Group, (2006). A randomized trial of diagnostic techniques for ventilator-associated pneumonia. - : N Engl J Med


Archive Article

Cover Media Content

Volume : 49 / No. : 2 / Pub. : 2013-04
  1. The effectiveness of topical garlic extract for methicillin resistant staphylococcus aureus treatment in burns
  2. Mycobacterium tuberculosis immunoglobulin m (igm) and immunoglobulin g (igg) survey in hiv-infected donors
  3. Comparing the effect of red yeast rice, date palm, and guava leaf extract on thrombocyte and megakaryocyte count in thrombocytopenic white rats
  4. Maternally inherited nucleotide variation of mitochondrial dna (mtdna) d-loop region 126 pb (nt: 34-159 hvs ii) in madurese individuals
  5. Rapid culture method using biphasic media on bacteriologic diagnosis to detect mycobacterium tuberculosis for determining pulmonary tuberculosis
  6. Effect of carbide and cacl2 concentration on vitamin c contents of banana kepok
  7. Direct cloning of melanoma antigen 1 (mage-1) and the e2 gene of hog cholera virus (hcv) from the blunt end pcr product using pet 101/d-topo
  8. Quantitation of microorganisms in lower respiratory tract secretions
  9. Bmd-qus assessment result appropriates to diagnose osteoporosis
  10. Acid-fast bacteria m. leprae examination in leprosy patients before and after treatment
  11. Diagnostic role of intra operative ovarian cyst fluid cytology in determining malignancy
  12. Correlation between adolescents' knowledge on drugs hazard and their behavior in drug abuse in tulungagung
  13. Basal insulin as add-on therapy to oral glucose-lowering drugs in insulin naive patients with type 2 diabetes mellitus in primary public health center