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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 1 / Published : 2011-01

TOC : 2, and page :6 - 9

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Original Article :

Sex determinant of human hair with dna analysis method using polymerase chain reaction (pcr)

Author :

  1. Ahmad Yudianto*1
  2. Nabil A Bahasuan*2
  1. Department of Forensic Medicine & Medicolegal Faculty of Medicine Airlangga University
  2. Department of Forensic Medicine & Medicolegal Faculty of Medicine Airlangga University

Abstract :

The identification of victims and suspects in criminal cases is increasing the quantity or quality. Environmental factors that affect DNA degradation that can be fast or slow, depending on factors that influence the occurrence and timing of exposure. DNA damage is divided into two types: from within, for example due to reactive oxygen species (ROS), and from outside, such as temperature and humidity. Evidence at the scene plays an important role in identification. Hair can be used in the determination of race, sex, blood type. But until now the examination through the hair as forensic identification of alternatives, not much is known. This study aims to find out the examination of hair as an alternative material with the identification of forensic DNA profiling. The results of this study, levels of DNA sample that is 20 and 35 ul/ml. On the visualization of agarose electrophoresis using polyacrylamide gel composit be determined whether the bands that appear 212 bp to 380 bp amelogenin X and Y for amelogenin, by drawing a line in the direction of the sample ribbon marker 100 bp. At sample number 1 there are two bands which is at 212 bp and 380 bp in the sample number 1 is the male sex (XY) while in sample number 2 was only one band at 212 bp so that the female gender (XX).

Keyword :

Sex determinant, Amelogenin, PCR,


References :

  1. Atmaja DS, (2005). Peranan sidik jari DNA pada bidang kedokteran forensic; Materi Workshop DNA fingerprinting . Yogyakarta : Universitas Gadjah Mada
  2. Hofreiter M, Serre D, Poinar HN, Kuch M, Paabo S, (2001). Ancient DNA. - : Nature Reviews Genetics
  3. Butler JM, et al. , (2004). The Development of Reduced Size STR Amplicons as Tools for Analysis of Degraded DNA. - : National Institute of Standards and Technology


   


Archive Article

Cover Media Content

Volume : 47 / No. : 1 / Pub. : 2011-01
  1. Evaluation of burn unit gbpt performance from 2006-2008. a retrospective study
  2. Sex determinant of human hair with dna analysis method using polymerase chain reaction (pcr)
  3. Protein kinase c: after the combination of metformin and hyperbaric oxygen therapy in type 2 diabetes patients
  4. Effectiveness omeprazole and lansoprazole in dyspepsia patient with nepean dyspepsia index
  5. Decrease of cross shift and cross week blood cholinesterase activity in pesticide sprayer of onion farmers
  6. Identification of mycobacterium tuberculosis adhesion protein in sputum of tuberculous patients
  7. Correlation of histopathological skin biopsies with clinical diagnosis in leprosy
  8. Review article: reducing maternal mortality rate (mmr) and infant mortality rate (imr): non institutional delivery or institutional delivery?
  9. Maternal risk factors of premature delivery in dr soetomo hospital
  10. Citric acid reduces the content of pb and cd of kupang beras (corbula faba)
  11. Difficulty in achieving hemodialysis adequacy in hemodialysis unit, dr. soetomo hospital, surabaya, indonesia
  12. Acute toxicity test of bark and stem ethanol extract of sopang (caesalpinia sappan linn) by brine shrimp lethality test
  13. Penile length of newborn infants in dr. soetomo hospital surabaya. a preliminary study