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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 41 / No. 2 / Published : 2005-04

TOC : 4, and page :90 - 95

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Original Article :

Spermatozoa motility and morphological recovery process in mice (mus musculus) after the induction of 2-methoxyethanol

Author :

  1. Alfiah Hayati *1
  2. Any Devi Rahmaninta *2
  3. IB Rai Pidada*3
  1. Department of Reproductive Biology Airlangga University School of Mathematics and Natural Sciences
  2. Department of Reproductive Biology Airlangga University School of Mathematics and Natural Sciences
  3. Department of Reproductive Biology Airlangga University School of Mathematics and Natural Sciences

Abstract :

This study used 30 male BALB/C mice aged 8 - 9 weeks with bodyweight of 28 - 30 grams. These experimental animals were divided into 6 groups, each comprising 5 mice. Group K1 was given with 2-ME of 200 mg/kg intraperitoneally every day for three weeks. The groups P1, P2, P3 and P4 were given with 2-ME, also for three weeks, and the administration was subsequently ceased for 1, 2, 3, and 4 weeks, respectively. Group K0 (control) was injected with saline solution. Spermatozoa were taken from epididymal cauda. Observation was carried out for the motility of 100 spermatozoa with types A, B, C or D (WHO, 1992), as well as to normal and abnormal morphology in head and tail, and combination of head and tail. Data were tested using one-way variant analysis. Results showed that the administration of 2-ME every day for 3 weeks resulted in azoospermia, so that sperm motility and morphology was not observable. However, by providing recovery period from 1 to 4 weeks, sperm motility and morphology was found to recover into normal. There was a significant difference in sperm motility and morphology after treatment and recovery. As conclusions, 2-Methoxyethanol given to male mice for 3 weeks may result in azoospermia, and the effect of the length of cessation period of 2-ME administration increases the recovery of sperm morphology and motility.

Keyword :

2-methoxyethanol, sperm motility and morphology,


References :

  1. Albert BD, Bray J, Lewis M, Raff K, Robert JD, Watson, (1989). Molecular Biology of The Cell . New York : Garland Publishing, Inc
  2. Berndtson WE, Foote RH, (1997). Disruption of spermatogenesis in rabbits consuming ethylene glycol monomethyl ether . - : Reproductive Toxicology


   


Archive Article

Cover Media Content

Volume : 41 / No. : 2 / Pub. : 2005-04
  1. Editorial vol 41 no 2 2005
  2. Opinion: bioethics. its role in research
  3. Gastroprotective effect of curcumine on ethanol-induced gastric mucosal lesions in rats
  4. Spermatozoa motility and morphological recovery process in mice (mus musculus) after the induction of 2-methoxyethanol
  5. Nutritional and immune effects of early enteral feeding supplemented by arginine, glutamine and omega 3 fatty acid in critically ill patients
  6. The comparison of the effectiveness between 2.5% povidone-iodine eye drop and 1% tetracycline eye ointment on bacterial ophthalmia neonatorum prophylaxis
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