UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 42 / No. 1 / Published : 2006-01

Order : 5, and page :28 - 32

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Original Article :

The 2-methoxyethanol toxicity towards the structures of plasma membrane and mitochondria of the rat’s spermatozoa

Author :

  1. Alfiah Hayati*1
  2. Soesanto Mangkoewidjojo*2
  3. Aucky Hinting*3
  4. Sukarti Moeljopawiro*4
  1. Department of Reproductive Biology, Airlangga University School of Mathematics and Natural Sciences
  2. Department of Clinical Pathology, Gadjah Mada University School of Veterinary Medicine
  3. Department of Medical Biology, Airlangga University School of Medicine
  4. Department of Biochemistry, Gadjah Mada University School of Biology

Abstract :

2-Methoxyethanol (2-ME) is characterised as a toxic and teratogenic compound in which the exposure to it can lead to increased incidences of embryonic death or damage, teratogenesis, or growth retardation. Epidemiologic studies have been conducted on a certain group of populations where it was shown that 2-ME can result abnormalities from olygospermia to azoospermia. The main reason for this research was to understand how this compound could affect the ultrastructures of spermatozoa membrane and mitochondria. Therefore, an experiment has been performed on 10 samples of male rats where the first five were injected with 0.2ml of 200 mg/kg of 2-ME, 6 days per week within 2 weeks. The rest were taken as a control group and were injected with 0.2ml of physiological electrolytes for the same duration as the first group. After the experiment was over, the samples were anesthetised with chloroform to perform a necropsy where the spermatozoa were taken from the caudal epididimis and vas deferens by using a flushing method. Spermatozoa were fixed with glutaraldehyde so that the structures could be examined thoroughly and it was observed by using an electron microscope transmission. The results showed that there were differences of the ultra structures between the tested rats and the control group. The exposure could damage the plasma membrane of the head and also the middle piece and also resulted in vacuolated mitochondria at the middle piece. The conclusion is that exposure of 2- ME can cause damage in the plasma membrane and mitochondria of the rats’ spermatozoa.

Keyword :

2-Methoxyethanol (2-ME), ultra structures, spermatozoa,


References :

RJ Aitken,(2000) Possible redox regulation of sperm motility activation no info : Jur of Andrology

HO Goyal,(2001) Diethylstilbestrol-treated adult rats with altered epididymal sperm numbers and sperm motility parameter but without alteration in sperm production and sperm morphology no info : Biology of Reproduction

TD Braden,(2001) Diethylstilbestrol-treated adult rats with altered epididymal sperm numbers and sperm motility parameter but without alteration in sperm production and sperm morphology no info : Biology of Reproduction

M Mansour,(2001) Diethylstilbestrol-treated adult rats with altered epididymal sperm numbers and sperm motility parameter but without alteration in sperm production and sperm morphology no info : Biology of Reproduction

CS William,(2001) Diethylstilbestrol-treated adult rats with altered epididymal sperm numbers and sperm motility parameter but without alteration in sperm production and sperm morphology no info : Biology of Reprodu



Archive Article

Cover Media Content

Volume : 42 / No. : 1 / Pub. : 2006-01
  1. Toxoplasma Gondii Infection Elicits The Increase Of Fas Expression On The Trophoblast Associated With The Increase Of Trophoblast Apoptosis
  2. Effect Of Laser At Pishu Point On Amount And Function Of Pancreatic β Cells (rattus Norvegicus) Injected By Streptozotocin
  3. The Effects Of Unilateral Urethral Obstruction On Tubular Cells Apoptosis:an Experimental Study In Rabbits
  4. Antimicrobial Activities Of Andrographolide And Propolis Against Intracellular Mycobacterium Tuberculosis Phagocytosed By Monocytes Derived Macrophages
  5. The 2-methoxyethanol Toxicity Towards The Structures Of Plasma Membrane And Mitochondria Of The Rat’s Spermatozoa
  6. Genotyping Of Extended Spectrum Β-lactamase Escherichia Coli Strains From Clinical Specimens
  7. Multiresistance Pattern Of Extended Spectrum β-lactamase (esbl) – Escherichia Coli And Klebsiella Pnemoniae Strains
  8. Fev1-reversibility In House-dust Immunotherapy Compared With Inhaled Corticosteroid In The Treatment Of Childhood Asthma
  9. Trachoma In Pondok Pesantren Salafiyah Syafiiyah, Sukorejo, Situbondo, East Java. A Case Study
  10. Review Article : Depression In Post-myocardial Infarction Patients
  11. Review Article And Clinical Experience: The Mets: One Of The Major Threat To Human Health

Information FMI