UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 43 / No. 2 / Published : 2007-04

Order : 6, and page :93 - 98

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Original Article :

The determination antipyrine on plasma by spectrophotometer ultra violet-visible

Author :

  1. Nuraini Farida*1
  2. Bambang Hermanto*2
  3. M Lazuardi*3
  1. Department of Medical Pharmacy Airlangga University School of Medicine
  2. epartment of Medical Pharmacy Airlangga University School of Medicine
  3. Airlangga University School of Veterinary Medicine

Abstract :

A spectrophotometer method of antipyrine has been used for the determination of analgesic antipyretic drug in human plasma. The objectives of this research were to obtained a rapid and validate new method for routine. The spectronic system was used UV detection at 230 nm for analyzed antipyrine in aqua pro injection. The sample pretreatment is a simple deproteination step by an organic solvent. The 1-octanosulphonic acid sodium salt as a counter-ion in the adsorption-partition phase system is added in order to increase the recovery of the almost complete protein-bound antipyrine. The critical value of /t / at the 5% confidence level indicates that there is no systematic error in the linearity proposed method. Recoveries for these seven serial concentrations were obtained at ranging 86.010 to 88.069%. The coefficient variations of precision at plasma concentration up to 50 ppm were obtained at ranging 0.433 to 3.871%. The minimum detectable concentration in aqueous was obtained at 2.5 µg.ml-1. This method was simple and accurate for using a routinely analyzed in plasma level guided and suitable for therapeutics drug monitoring studies.

Keyword :

antipyrine, bio-analysis, spectrophotometer UV-Vis, plasma,


References :

K Akira,(1999) Direct Detection of Antipyrine Metabolites in Rat Urine by 13C Labeling and NMR Spectroscopy no info : Drug Metabolism and Disposition

AOAC-International,(2002) Official Methods of Analysis of AOAC International. (Agricultural Chemicals; Contaminants; Drugs. The Scientific Association AOAC no info : International Press

RA Branch,(1976) The clearance of antipyrine and indo cyamine green in normal subjects and in patent with chronic liver diseases no info : Clinical Pharmacology and Therapeutic

JA James,(1976) The clearance of antipyrine and indo cyamine green in normal subjects and in patent with chronic liver diseases no info : Clinical Pharmacology and Therapeutic

AE Read,(1976) The clearance of antipyrine and indo cyamine green in normal subjects and in patent with chronic liver diseases no info : Clinical Pharmacology and Therapeutic

J Chamberlain,(1987) Analysis of Drug in Biological Fluids Boca Raton Florida : CRC Press Inc





Archive Article

Cover Media Content

Volume : 43 / No. : 2 / Pub. : 2007-04
  1. Detection Of Mycobacterium Leprae Dna In Blood Of The Subclinical Leprosy
  2. Correlation Between Mutant P53 Protein Expression And Histopathological Grading In Astrocytoma Patients
  3. Histopathological Feature Of The Tumor Of Central Nervous System At Airlangga University School Of Medicine, Dr Soetomo Hospital, Surabaya, In 2001-2003
  4. Exercise Diastolic Blood Pressure As An Indicator Of Endothelial Function Among Subjects With Normal And Low Ankle-brachial Index
  5. Effect Of Artificial Unilateral Ureter Total Obstructon On Serum Cystatin-c Levels In Oryctalagus Cuniculus
  6. The Determination Antipyrine On Plasma By Spectrophotometer Ultra Violet-visible
  7. Lumbar Microdiscectomy: Technique, Advantages And Outcomes. Surabaya Experience On 83 Patients
  8. Case Report: Successful Treatment Of Stevens Johnson Syndrome-toxic Epidermal Necrolysis With Intravenous Immunoglobulin
  9. Review Article: Hysterosalpingography As A Diagnostic Test Of Female Infertility
  10. Review Article: Heart Rate Variability. A Remarkable Tool To Assess Sympathovagal Balance
  11. Review Article And Clinical Experience: Hypertension In Diabetic Nephropathy. The Five “satan Circles”, Dha-licoar, The Roles Of Valsartan

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