Folia Medica Indonesiana
ISSN 0303-7932
Vol. 38 / No. 2 / Published : 2002-04
Related with : Scholar Yahoo! Bing
Original Article :
The effect of vitamin a on the immunomodulation of mucosal immune response an experimental animal model
Author :
Abstract :
Vitamin A deficiency may affect the immune system leading to decreasing capacity of immune response, both systemic and mucosal arm. The consequences of vitamin A deficiency may lead to be more sensitive to contract infection especially at the mucosal site. Most of infections go through mucosa as port of entry, it means that the body has to posses an appropriate mucosal immune system for preventing infection against pathogen. In mucosal immune system T cell CD4 and CD8 as part of immunocompetent cells play prominent roles in the process of immune response against enteropathogen. The objective of the study is to elucidate the effect of vitamin A as an immunomodulator in mucosal immune response of vitamin A deficient rats, expressed as ratio CD4/CD8. Material and methods. An experimental animal model using male Wistar rats was created. The age of rats was more than three weeks after being weaned from the mother. Vitamin A deficient rats population was made by using special established protocol developed by Sirisinha. After randomization the study population was divided into three groups, vitamin A deficient, control, and vitamin A deficient treated group. Induction of immune response was done by administration of lipopolysaccharide (LPS). Serum level of vitamin A was determined by high performance liquid chromatography (HPLC). Immunohistochemistry by monoclonal antibody technology was used to express T cell CD4 and CD8 in the Peyer’s patch as an inductive and lamina propria as an effector site of the intestinal mucosa. Results: The mean level of vitamin A in vitamin A deficient, control, and vitamin A deficient treated group were 8.65 mcg/dl, 52.40 mg/dl, and 39.60 mcg/dl, there were significant difference with F value 0.0000. In the inductive site, the ratio of CD4/CD8 is 1.07 in the vitamin A deficient group, and after treated with vitamin A the CD4/CD8 ratio increased to 1.60. While in the effector site the increasing ratio is more than twice, from 0.86 become 2.21. In the group of unexposed to LPS in the inductive site, there is no significant change of CD4/CD8 ratio, value of 0.86 in the vitamin A deficient group and 0.81 in the group vitamin A deficient treated group. In the effector site there is no significant elevation of the ratio of CD4/CD8 from 1.05 in the A deficient group become 1.22 in the vitamin A deficient treated group. Conclusion: From this study we conclude that vitamin A has an immunomodulator effect on the mucosal immune response (MIR).
Keyword :
vitamin A, immunomodulator, mucosal immune response, mucosal immune status,
References :
Archive Article
| Cover Media | Content |
|---|---|
 Volume : 38 / No. : 2 / Pub. : 2002-04 |
|
