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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 42 / No. 1 / Published : 2006-01

TOC : 3, and page :15 - 21

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Original Article :

The effects of unilateral urethral obstruction on tubular cells apoptosis:an experimental study in rabbits

Author :

  1. B Daryanto*1
  2. DM Soebadi*2
  3. Soetojo*3
  4. Sunaryo Hardjowijoto*4
  1. Department of Urology Airlangga University School of Medicine Dr Soetomo Teaching Hospital Surabaya, Indonesia
  2. Department of Urology Airlangga University School of Medicine Dr Soetomo Teaching Hospital Surabaya, Indonesia
  3. Department of Urology Airlangga University School of Medicine Dr Soetomo Teaching Hospital Surabaya, Indonesia
  4. Department of Urology Airlangga University School of Medicine Dr Soetomo Teaching Hospital Surabaya, Indonesia

Abstract :

To explain effects of unilateral urethral obstruction on tubular cells apoptosis as the mechanism of renal dysfunction. Forty-eight rabbits (Oryctalagus cuniculus) underwent distal unilateral urethral obstruction. The experimental animals were divided into 6 groups: 2 groups had sham operation, which served as control, 2 groups had left urethral partial obstruction, 2 groups had left urethral total obstruction. Tubular cells apoptosis of the ipsilateral kidney were analyzed 7 days and 14 days after obstruction by means of immunohistochemical method. T analysis revealed no different tubular cells apoptosis of the ipsilateral kidney between partial urethral obstruction groups and control groups at 7 and 14 days (p = 0.085 and p = 0.252), but there were differences in total urethral obstruction groups at 7 and 14 days. ANOVA analysis revealed that in unilateral urethral obstruction there were different tubular cells apoptosis of the ipsilateral kidney in control, partial and total urethral obstruction groups at 14 days (p = 0.0001). There were different tubular cells apoptosis of the ipsilateral kidney between control and partial urethral obstruction groups, control and total urethral obstruction groups (p = 0.0001) at 7 days. Tubular cells apoptosis of the ipsilateral kidney in total urethral obstruction groups were different at 7 and 14 days (p = 0.012), and not different in partial urethral obstruction groups at 7 and 14 days (p = 0.252). There were no different tubular cells apoptosis of the ipsilateral kidney in partial and total urethral obstruction at 7 days but the difference was found at 14 days.

Keyword :

Unilateral urethral obstruction, tubular cells apoptosis, ipsilateral kidney,


References :

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  2. Badawy, (1999). Urethral obstruction transiently upregulates the expression of heparin-binding EGF like growth factor in the mouse kidney. no info : J. Urol
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  5. A Erbagci, (2002). Evaluation of the tubular apoptosis in unilateral obstruction in rabbit model. Kolejtepe, Turkey : University of Gazantiep, Sahinbey Hospital
  6. JM Fitzpatrick, (1999). Structure and Function of the Upper Urinary Tract in Health and Disease. Oxford : Isis Medical Media Ltd.
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  8. HT Nguyen, (2000). Heparin Binding EGF-Like Growth Factor Is Up-Regulated in the Obstructed Kidney in a Cell and Region-Specifik Manner an Acts to Inhibit Apoptosis. no info : American Journal of Pathology
  9. Kuntoro, (1994). Materi Pokok Pelatihan Metodologi dan Statistik. Surabaya : Lembaga Penelitian UNAIR
  10. G Kroeme, (2000). Mitochondria Control of Cell Death. no info : Nature Medicine


   


Archive Article

Cover Media Content

Volume : 42 / No. : 1 / Pub. : 2006-01
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  3. The effects of unilateral urethral obstruction on tubular cells apoptosis:an experimental study in rabbits
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