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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 38 / No. 1 / Published : 2002-01

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Original Article :

The influence of vitamin a deficiency on the expression of antigen presenting cells in rat mucosal immune response

Author :

      Abstract :

      APC as immunocompetent cell plays an important role on the initiation of mucosal immune response. Vitamin A deficiency may affect expression population of APC. Decreasing of APC population in mucosal sites may reduce the quality, the initiation of immune response and resulting in increasing risk for mucosal infection. The objective of the study is to elucidate the effect of vitamin A deficiency on the expression of APC in the mucosal immune response. Experimental animal model with use of male weaned Wistar rats were created. Vitamin A deficient rats were made by using a special established protocol developed by Sirisinha. After randomization the study population was divided into three groups, vitamin A deficient, control, and vitamin A deficient treated group. Induction of immune response was done by administration of LPS. Serum level of vitamin A was measured by high performance liquid chromatography (HPLC). Immunohistochemistry by monoclonal antibody was used to express APC in the Peyer’s patch as an inductive and lamina propria as an effector site of the intestinal mucosa. Results. The mean level of vitamin A in vitamin A deficient, control, and vitamin A deficient treated group were 8.65 g/dl, 52.40 g/dl, and 39.60 g/dl, there was significant different with F value 0.0000. In exposed to LPS (MIR) in the inductive site there was significant different of APC between vitamin A deficient group and vitamin A deficient treated group with p = 0.042. In the effector site there was no significant different between vitamin A deficient group and vitamin A deficient treated group, with p =: 0.486. In unexposed group to LPS (MIS) in the inductive site there was no significant different of APC between vitamin A deficient group and vitamin A deficient treated group with p = 0.99. In the effector site there was no significant different between vitamin A deficient group and vitamin A deficient treated group with p = 0.035. Conclusion. Vitamin A deficiency affect MIS in terms of decreasing APC in the inductive site and effector site, and may reduce the induction of MIR. However this abnormality is reversible.

      Keyword :

      vitamin A deficiency, antigen presenting cell (APC), mucosal immune response (MIR), mucosal immune status (MIS).,


      References :



           


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