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Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 4 / No. 1 / Published : 2015-06

TOC : 8, and page :44 - 50

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Original Article :

Effect of guava leaves (psidium guajava l.) extract on total and differential count of leucocytes in mice (mus musculus) exposed to heat stress

Author :

  1. Shervida Rismawati*1
  2. Dewa Ketut Meles*2
  3. Arimbi*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study was to find out the effect of guava leaves (Psidium guajava L.) extract to maintaining total and differential count of leucocytes as innate immunity in mice (Mus musculus) exposed to heat stress. This study was using thirty mice divided into five groups of experiment, that were K(-), P0, P1, P2, and P3. All group of experiments except K(-), received heat stress exposure twice daily for 30 minute at temperature 39-40 oC with 15 minutes interval. P1, P2 and P3 were treated with oral administration of different dose of guava leaves extract. P1 received 4.028 mg/20 g BW/day guava leaves extract; P2 received 7.28 mg/20 g BW/day guava leaves extract; P3 received 8.445 mg/20 g BW/day guava leaves extract respectively. The extract given for 10 days and heat stress exposure given for the last three days. Total and differential count of leucocytes were measure on the last day of experiments after blood sample collected intra cardiac with inhalation anesthesia. Statistical analyses was done by one way ANOVA followed by Tukey Test. The result of the research showed there were significant differences (p<0.05) on  total leucocytes neutrophilia and lymphocytes and there were no significant difference (p>0.05) on eosinophiles, basophiles, neutrophiles and monocytes. The conclusion of the treatment showed that P3 was the best group experiments that maintaining total leucocytes, neutrophiles and lymphocytes in mice exposed to heat stress.  

Keyword :

Guava leaves (Psidium guajava L.), mice (Mus musculus), heat stress, total and differential count of leucocytes,

References :

  1. Hazel, L And K. A. Roebuck. , (2001). Oxidant stress and endothelial cell dysfunction. . 280: 719-741. : Am J Physiol cell Physiol
  2. Lakshmi B.V.S, Sudhakar M. . , (2009). Screening of Psidium guajava Leaf Extracts for Antistres Activity in Different Experi¬men-tal Animal Models. 1(6): 359-366 : Pharma-cognosy Research
  3. Neal, M. J. 2002. Neal, M. J.. , (2002). Medical Pharmacology at a Glance . . Fourth Eidtion: 72-73 : Wiley-Blackwell.


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2015-06
  1. Anthelmintic activity of bermuda grass (cynodon dactylon) ethanol extract on ascaridia galli by in vitro
  2. The effect combination of guava leaf (psidium guajava l.) and sambiloto leaf (andrographis paniculata) on total and diffcount leucocyte of mice (mus musculus) exposed to heat stress
  3. Effect of meniran (phyllanthus niruri, linn.) infusion on white pulp spleen diameter of vibrio anguillarum infected gouramy fish
  4. Dipping of meniran infusion (phyllantus niruri) on gourame (osphronemus gouramy) infected with vibrio anguillarum toward the level of hemoglobin, packed cell volume and number of erythrocytes
  5. Effect of propolis toward the number and type of blood leucocyte count of male mice (mus musculus)
  6. Effect of cigarette smoke on type ii pycnotic cells at intralveolar septa of mice treated by aromatherapy extract of hibiscus sabdariffa calyx
  7. Immunomodulator effect of sambiloto (andrographis paniculata) to total and differential counting of leucocytes in mice (mus musculus) exposed to heat stress
  8. Effect of guava leaves (psidium guajava l.) extract on total and differential count of leucocytes in mice (mus musculus) exposed to heat stress
  9. Most probable number (mpn) of escherichia coli isolated from water used by the food vendors around campus of veterinary medicine faculty of airlangga university
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