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Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 3 / No. 1 / Published : 2014-06

TOC : 5, and page :29 - 33

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Original Article :

Identification and isolation of influenza virus (h5) in pig in north sumatra

Author :

  1. Barcelona Bakkara*1
  2. Chairul Anwar Nidom*2
  3. Hasutji Endah Narumi*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study was to detect H5 subtype influenza viruses and seroprevalence on pigs in North Sumatra.  Identification of the virus conducted by collecting swab of the nasal cavity. The specimens  inoculated into Specific antibody negative (SAN) embryonated chicken eggs (9–11 days old). Allantoic fluid was harvested and tested by using haemaglutination (HA) test. Seroprevalence were tested by hemagglutination inhibition (HI) test. Based on HA test indicated there was no titer of haemagglutinin activity in all samples (0%). However There were 11 positive samples from 100 samples (11%) by HI test. Seroprevalence of each district were 11.11% in Karo, 21.74% in Deli Serdang and 0% in  Binjai.      

Keyword :

seroprevalence, influenza virus, Pig,


References :

  1. Rahardjo, J dan C.A. Nidom.. , (2004). Avian Influenza : Pencegahan, Pengendalian, dan Pemberan-tasannya. Hasil Investigasi Lapangan. .. Jakarta : Gita Pustaka.
  2. WHO. , (2014). Cumulative number of confirmed human cases for avian influenza A (H5N1) .. Amerika : reported toWHO,2003-2014.http:// www .who.int/influenza/human_animal_interface/EN_GIP_20140124CumulativeNumberH5N1cases.pdf.
  3. Xu, X., K. Subbarao., N.J. Cox., Y. Guot. , (1999). Genetic characterization of the pathogenicinfluenza A/ Goose/Guangdong/1/96(H5N1) virus: similarity of its hemag¬glutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong.. Hongkong : Virology 261 (1), 15–19.


   


Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-06
  1. Detection of avian influenza antibody h5 subtype on bearded pig (sus barbatus) in central kalimantan
  2. The effect of infusion of tekelan (chromolaena odorata l.) leaf to wound healing incision on mice (mus musculus) were infected by staphylococcus aureus
  3. Activity test of krisan flower (chrysanthemum morifolium ramat) exstract on stable fly (stomoxys calcitrans geof.) mortality by in vitro
  4. Epidemiology of avian influenza virus (h5) from duck outbreak in the 2012-2013 in east java
  5. Identification and isolation of influenza virus (h5) in pig in north sumatra
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