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Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 5 / No. 1 / Published : 2016-06

TOC : 5, and page :25 - 31

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Original Article :

Reactivity test of 30 kda protein aeromonas hydrophila, isolated by freshwater fish with indirect elisa

Author :

  1. Dwi Ratna Aristantya *1
  2. M. Gandul Atik Yuliani *2
  3. Endang Suprihati*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This study aimed to determine the reactivity of 30 kDa protein Aeromonas hydrophila. The reactivity is indicated by a binding between the antigen and antibody of rabbit (Oryctolangus cuniculus) that is based in the optical density score in indirect ELISA technique. The isolated Aeromonas hydrophila was characterized in previous research using Western-Blot and SDS-PAGE method, produced Aeromonas hydrophila protein that stated in 30 kDa has potential immunogenic. Three male rabbits immunized with 1 ml of adjuvant as control, 108 cfu/ml whole protein of Aeromonas hydrophila as P1 and 2.5x107 IU/ml of protein 30 kDA Aeromonas hydrophila as P2 respectively. Immunization was done four times with an interval of 14 days. Antibody titer tested by indirect ELISA technique from collected serum. The result showed that the antibody titer after immunization IV (1.550) higher than after immunization I (1.341) and pre-immune (1.219).

Keyword :

Aeromonas hydrophila, protein, immunization, ELISA,


References :

  1. Soeripto, (2002). Pendekatan Konsep Kesehatan Hewan Melalui Vaksinasi. 21(2): 48-55 : Jurnal Litbang Pertanian


   


Archive Article

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Volume : 5 / No. : 1 / Pub. : 2016-06
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  2. The effect repeated exposures artemisinin of white pulp diameter and spleen index on mice (mus musculus) which infected by plasmodium berghei
  3. The effect of ambon banana hump (musa paradisiaca var. sapientum) extract on histopathologic of rats (rattus norvegicus) jejunum inflammatory bowel disease
  4. Effects of propolis on histology profile of kidney in male mice (mus musculus)
  5. Reactivity test of 30 kda protein aeromonas hydrophila, isolated by freshwater fish with indirect elisa
  6. Antibacterial activity of bitter leaf extract (andrographis panicullata, nees) against staphylococcus aureus isolates field with in vitro method
  7. Effect extract spirulina platensis on kidney histopathology of gouramy fish (osphronemus gouramylac.) infected with aeromonas hydrophila
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  9. Effect of laserpuncture exposure on bl-18 acupunture point’s to the level of sgot and sgpt in white rat (rattus norvegicus) were induced by paracetamol
  10. The effect of crude arthrospira sp in layer feed to optical density and immunoglobulin a levels
  11. The immersion effect of spirulina platensis extract on hepatopancreas of carp fish (osphronemus gouramy) that infected aeromonas hydrophila
  12. The effect of green bitter melon fruit flesh (momordica charantia l.) extract on mice (mus musculus) estrous cycle which superovulated with pmsg and hcg