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Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 3 / No. 1 / Published : 2014-06

TOC : 7, and page :40 - 44

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Original Article :

Toxicity test of ethanol extract of red roses (rosa damascena mill) by brine shrimp lethality test (bslt) method

Author :

  1. Khusnul Rizal Hutabarat*1
  2. Eduardus Bimo Aksono H*2
  3. Ismudiono*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This study aimed to determine the acute toxicity test effects of ethanol extract of red roses using the Brine Shrimp lethality Test (BSLT) method and determine the LC50 values of ​​Artemia salina Leach larvae after the administration of ethanol extract of red roses. This research is an experimental post-test-only control group design. Experimental animals used 300 larvae Artemia salina Leach were divided into 5 groups. Each group consisted of 10 animals. Each group performed 5 repetitions trial. As the test material was ethanol extract of red roses given to the media solution containing the test animals. Final concentration of extract was 100%, 31.5%, 10%, 3%, 1% and 0% as control. Data obtained from counting the number of larvae that died 24 hours after treatment. Based on the data, LC50 basil leaf ethanol extract was determined by probit analysis using SPSS. The results of average value of larval mortality at concentration of 100%, 31.5%, 10%, 3%, 1% and 0% respectively were 10, 10, 10, 22, 13, 0. The higher concentration of the extract led to the higher number of larvae mortality. Results of probit analysis showed LC50 of the ethanol extract of red roses is 301834,69829 µg/ml. Ethanol extract of red roses (Rosa damascena Mill) in this study showed no potential for acute toxicity on the larvae of Artemia salina Leach indicated a price of LC50 > 1000 µg/ml according to the method of Brine Shrimp lethality Test (BSLT).    

Keyword :

Rosa damascena Mill, Brine Shrimp lethality Test, Artemia salina Leach, toxicity,

References :

  1. Cahyadi, R. , (2009). Uji Toksisitas Akut Ekstrak Etanol Buah Pare (Momordica charantia L.) Ter-hadap Larva Artemia salina Leach dengan Metode Brine Shrimp Lethality Test (BST). Laporan Akhir Penelitian Karya Tulis Ilmiah. . Semarang. : Fakultas Kedokteran. Universitas Diponegoro.
  2. Madhavi D. L., S. S Deshpande., D. K Salunkhe. , (1996). Food Antioxidants (Food science and technology). . New York : M. Dekker, cop.
  3. Satoh K, Ida Y, Sakagami H, Tanaka T, Fujisawa S. , (1998). Effect of antioxidants on radical intensity and cytotoxic activity of eugenol. . Amerika : Anticancer Research 1998. 18: 1549-52. Available from :


Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-06
  1. Detection of avian influenza antibody h5 subtype on bearded pig (sus barbatus) in central kalimantan
  2. The effect of infusion of tekelan (chromolaena odorata l.) leaf to wound healing incision on mice (mus musculus) were infected by staphylococcus aureus
  3. Activity test of krisan flower (chrysanthemum morifolium ramat) exstract on stable fly (stomoxys calcitrans geof.) mortality by in vitro
  4. Epidemiology of avian influenza virus (h5) from duck outbreak in the 2012-2013 in east java
  5. Identification and isolation of influenza virus (h5) in pig in north sumatra
  6. Effect of margarine and shortening trans fatty acid on rats (rattus norvegicus) hdl cholesterol level
  7. Toxicity test of ethanol extract of red roses (rosa damascena mill) by brine shrimp lethality test (bslt) method
  8. Effect of phyllanthus niruri linn extracts on blood glucose levels in diabetic rats (rattus norvegicus) induced by alloxan
  9. The effect of meniran extract (phyllanthus niruri linn.) against sgot and sgpt level of white rats (rattus norvegicus) induced by alcohol
  10. The effect of borax to duodenal histopathological changes in rats (rattus norvegicus)