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Jurnal Farmasi dan Ilmu Kefarmasian

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Vol. 1 / No. 2 / Published : 2014-12

TOC : 5, and page :55 - 58

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Original Article :

Efek apoptosis ekstrak etanol ganggang hijau (spirogyra sp.) terhadap ekspresi caspase-3 dan bcl-2 pada sel t47d

Author :

  1. Wahyu Widyaningsih*1
  2. Nina Salamah*2
  3. Dwi Fitriani*3
  4. Hari Susanti*4
  1. Fakultas Farmasi, Universitas Ahmad Dahlan, Jl Prof Soepomo, Janturan, Yogyakarta
  2. Fakultas Farmasi, Universitas Ahmad Dahlan, Jl Prof Soepomo, Janturan, Yogyakarta
  3. Fakultas Farmasi, Universitas Ahmad Dahlan, Jl Prof Soepomo, Janturan, Yogyakarta
  4. Fakultas Farmasi, Universitas Ahmad Dahlan, Jl Prof Soepomo, Janturan, Yogyakarta

Abstract :

Breast cancer is the leading cause of death in women in many parts of the world. Green algae              (Spirogyra sp.) Is one of the medicinal plants used in traditional medicine for the treatment of cancer. Green algae (Spirogyra sp.) has an active substance content in the form of melatonin. Melatonin is a compound that has been examined by researchers world sci as anticancer drugs and antioxidants. This study aims to determine the apoptotic effects of ethanol extract of green algae (Spirogyra sp.) On the expression of caspase-3 and Bcl-2 in T47D cells . Green algae powder was extracted by using a Soxhlet apparatus with 96% ethanol. This study used three groups: control cells, the concentration of ethanol extract of green algae 247.668 mg/ml and 123.834 mg/ml. To ensure the expression of caspase-3 and Bcl-2 test indirect immunocytochemistry. The results show there is an increased expression of caspase-3 and decrease in Bcl-2 when compared with control cells at a concentration of 123.834 mg/ml The ethanol extract of green algae (Spirogyra sp.) Has effect as an inducer of apoptosis at a concentration of 123.834 mg/ml .

Keyword :

Green algae, Spirogyra sp., caspase-3, Bcl-2, T47D cells, immunocytochemistry,


References :

  1. Prabowo A, (2009). Pemanfaatan phytomelatonin ganggang hijau (Spirogyra sp.) sebagai cancer activity inhibitor dari induksi logam berat. Yogyakarta : Universitas Ahmad Dahlan
  2. Lumongga F, (2008). Apoptosis. Medan : Departemen patologi anatomi fakultas kedokteran universitas sumatera utara
  3. Katzung BG, (1998). Basic and Clinical Pharmacology, Edisi VI, diterjemahkan oleh Binawati HK, Budi I, dkk. Jakarta : Buku Kedokteran ECG
  4. Hanahan D, Weinberg PA, (2000). The Hallmarks of Cancer Cell, Departement of Biochemistry And Biophysics And Hormone Research Institute. San Fransisco : University Of California
  5. Albert B, Julian L, Martin R, Keith R, Peter W, (2008). Molecular biology of the cell, Fifth Edition. Oxford : Garland Science


   


Archive Article

Cover Media Content

Volume : 1 / No. : 2 / Pub. : 2014-12
  1. Evaluasi mutu pelayanan di apotek komunitas kota kendari berdasarkan standar pelayanan kefarmasian
  2. Permasalahan terkait obat antihipertensi pada pasien usia lanjut di poli geriatri rsud dr.soetomo, surabaya
  3. Studi in silico gendarusin a, b, c, d, dan e untuk prediksi absorbsi dan aktivitas terhadap hialuronidase (ec 3.2.1.35)
  4. Pengaruh sistem miroemulsi tipe w/o terhadap karakteristik sediaan dan pelepasan natrium diklofenak (perbandingan konsentrasi surfaktan (span 80-tween 80): kosurfaktan (etanol 96%) = 6:1 dalam basis gel hpmc 4000)
  5. Efek apoptosis ekstrak etanol ganggang hijau (spirogyra sp.) terhadap ekspresi caspase-3 dan bcl-2 pada sel t47d
  6. Perbandingan aktivitas antioksidan ekstrak aseton tomat segar dan pasta tomat terhadap 1,1-diphenyl-2-picrylhidrazyl (dpph)
  7. Uji aktivitas ekstrak biji sirsak (annona muricata l.) terhadap sel kanker mamalia secara in vitro