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Jurnal Farmasi dan Ilmu Kefarmasian

ISSN

Vol. 1 / No. 2 / Published : 2014-12

TOC : 7, and page :63 - 66

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Original Article :

Uji aktivitas ekstrak biji sirsak (annona muricata l.) terhadap sel kanker mamalia secara in vitro

Author :

  1. Lusiana Arifianti*1
  2. Sukardiman*2
  3. Herra Studiawan*3
  4. Rakhmawati*4
  5. Lulus Megawati*5
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Dosen Fakultas Farmasi
  4. Dosen Fakultas Farmasi
  5. Mahasiswa Fakultas Farmasi

Abstract :

Cancer is the process of body cells proliferation that is uncontrolled. Treatment of cancer such as surgery, radiotherapy and chemotherapy have many side effects, therefore natural anticancer is needed. The soursoup (Annona muricata L.) is a traditional medicinal plant that is empirically used for anti-inflammatory and antitumor by Indonesian. The purpose of this study is to determine the cytotoxic effect of Annona muricata ‘s seed extract against T47D, HeLa, WiDr and Raji mammalian cancer cell lines. The cytotoxic test performed by MTT assay. The parameter obtained from the cytotoxic test was IC50,  that produce inhibitory concentration of cancer cells by 50%. The results showed that  A. muricata seed extract has a cytotoxic activity with IC50 were 20.36 ± 1.58 µg/mL; 8.91 ± 4.49  µg/mL;  40.06 ± 3.12  µg/mL and 11.24 ± 4.53 µg/mL for T47D, HeLa, WiDr and Raji cells respectively. It can be concluded that the A. muricata seed extract was potential to be delevoped as anticancer agent especially on servics cancer.

Keyword :

Annona muricata Linn, MTT assay, WiDr, HeLa, Raji,


References :

  1. Evira D, (2013). The Miracle of Fruits. - : Gramedia Pustaka
  2. Alali FQ, Liu XX, McLaughlin JL, (1999). Annonaceous acetogenins: recent progress. - : Journal of Natural Product
  3. Ukwubile CA, (2012). Phytochemical Screening and Anti-Ovarian Cancer Properties of Annona muricata Linn. (Annonaceae) Seed Ethanol Extract. - : International Journal of Pharmaceutical Frontier Research
  4. Karimian H, Moghadamtousi SZ, Kadir HA., Rouhallahi E, Paydar M, Fadaeinasab M, (2014). Annona muricata Leaves induced G1 cell cycle arrest and apoptosis through mitochondrial-mediated pathway in human HCT-116 and HT-29 colon cancer cells. - : Journal of Ethnopharmacology
  5. Moghadamtousi SZ, Kadir HA, Rouhallahi E, Paydar M, Fadaeinasab M, Karimian H, (2014). Annona muricata Leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-kB. - : BMC Complementary and Alternative Medicine


   


Archive Article

Cover Media Content

Volume : 1 / No. : 2 / Pub. : 2014-12
  1. Evaluasi mutu pelayanan di apotek komunitas kota kendari berdasarkan standar pelayanan kefarmasian
  2. Permasalahan terkait obat antihipertensi pada pasien usia lanjut di poli geriatri rsud dr.soetomo, surabaya
  3. Studi in silico gendarusin a, b, c, d, dan e untuk prediksi absorbsi dan aktivitas terhadap hialuronidase (ec 3.2.1.35)
  4. Pengaruh sistem miroemulsi tipe w/o terhadap karakteristik sediaan dan pelepasan natrium diklofenak (perbandingan konsentrasi surfaktan (span 80-tween 80): kosurfaktan (etanol 96%) = 6:1 dalam basis gel hpmc 4000)
  5. Efek apoptosis ekstrak etanol ganggang hijau (spirogyra sp.) terhadap ekspresi caspase-3 dan bcl-2 pada sel t47d
  6. Perbandingan aktivitas antioksidan ekstrak aseton tomat segar dan pasta tomat terhadap 1,1-diphenyl-2-picrylhidrazyl (dpph)
  7. Uji aktivitas ekstrak biji sirsak (annona muricata l.) terhadap sel kanker mamalia secara in vitro