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Jurnal Ilmiah Biologi FST

ISSN 2303-3428

Vol. 1 / No. 1 / Published : 2013-01

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Original Article :

Pengaruh periode subkultur terhadap kadar saponin akar rambut tanaman ginseng jawa (talinum paniculatum gaertn.)

Author :

  1. Aila Ikhtimami*1
  2. Hery Purnobasuki*2
  3. Y. Sri Wulan Manuhara*3
  1. -
  2. -
  3. -

Abstract :

The aim of this study was  to determine the effect of subculture period on dry weight and saponin content  of  ginseng jawa  (Talinum paniculatim  Gaertn.)  hairy root. This study used transformated hairy root  of  T.paniculatum  leaf  explants which has been inserted  by  T-DNA plasmid of Agrobacterium rhizogenes  and the media were  semisolid MS medium without plant growth regulators as subculture medium. The roots were treated by subculture periods  (0, 2, 3, 4 weeks) and were cultured for 10 weeks.  Datas were obtained as fresh weight  root, dry weight  root, and saponin content at the end of subculture period. Dry weight data analysis used one-way ANOVA followed  up with  LSD  test  (5% significance level). Saponin content  were analyzed by semi-quantitative descriptive with Thin Layer Chromatography (silica gel GF254 ).  The results showed that the  treatment of various subculture  periods had  significant influence in increasing  dry weight  of hairy root  and 2-weeks  subculture  period treatment produced  the highest  dry weight of hairy root with  0,0229±0,0031  g dry weight.  The result of saponin content  on Thin  Layer Chromatography test showed that  the treatment of various subculture  periods had effect on saponin content of hairy root and the highest saponin content obtained in without subculture (0 week) treatment with spot width 34±2,83 mm 2 /0,04 dry weight.  The conclusion of this study were  the treatment of various periods of subculture  had  significant effect on the  T. paniculatum  hairy root dry weight with the highest dry weight obtained at 2-weeks subculture period treatment  with 0,0229±0,0031  g dry weight  and various of subculture periods  had effect on saponin content of T. paniculatum hairy root, the highest saponin content obtained  in  without subculture  treatment  with  spot width  34±2,83 mm 2/0,04 dry weight.

Keyword :

Hairy root, ginseng jawa (Talinum paniculatum Gaertn.), subculture, saponin,


References :

  1. Hendaryono, D.P.S, dan A. Wijayani, (1994). Teknik Kultur Jaringan : Pengenalan dan petunjuk Perbanyakan Tanaman Secara Vegetatif-Modern, Kanisius. yogyakarta : -
  2. Grierson, D., and S. N. Covey, (1998). Plant Moleculer Biology, 2 nd edition, . New York : Blackie, Chapmann & Hall, New York, 141-147
  3. Bhojwani, S.S and Razdan M.K, (1996). Plant Tissue Culture: Theory and Practice, a Revised Edition. - : Elsevier
  4. Heyne K, (1987). Tumbuhan berguna Indonesia. - : Litbang Kehutanan Dep Pertanian RI.
  5. Jay, V, S. Genestier, J.C. Courdoroux, (1992). Bioreactor Studies on The Effect of Dissolved Oxygen Concentrations on Growth and Differentiation of Carrot (Daucus carota L.). - : Cell Cultures, Plant Cell Reports 11


   


Archive Article

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Volume : 1 / No. : 1 / Pub. : 2013-01
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  12. Pengaruh periode subkultur terhadap kadar saponin akar rambut tanaman ginseng jawa (talinum paniculatum gaertn.)
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