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Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 8 / No. 2 / Published : 2016-11

TOC : 5, and page :94 - 108

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Original Article :

Activity enzymatic of isolate lactic acid bacteria from the digestive tract of mud crab (scylla spp.) as a candidate probiotics

Author :

  1. Heru Pramono*1
  2. Pipin Suciati*2
  3. Wahju Tjahjaningsih*3
  4. Endang Dewi Masithah*4
  1. Dosen Fakultas Perairan dan Kelautan
  2. Mahasiswa Fakultas Perairan dan Kelautan
  3. Dosen Fakultas Perairan dan Kelautan
  4. Dosen Fakultas Perairan dan Kelautan

Abstract :

digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can beused as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze apolypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such asproteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolatesfrom the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This researchuses descriptive method such as enzymatic activity and biochemical characterization of isolates of lacticacid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish.The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genusPediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to thegenus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolyticactivity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolyticactivity, amylolytic, and lipolytic.

Keyword :

Mud crab, lactic proteolytic, amylolytic, lipolytic,


References :

  1. S. Sumarsih,, (2010). 2010. Pengaruh Aras Starter Lactobacillus sp. terhadap Performa Mikrobiologi Silase Ikan Dilihat dari Total Bakteri, Bakteri Asam Laktat dan Fungi. Jurnal Kesehatan. 3 (1) : 43-50. : urnal Kesehatan


   


Archive Article

Cover Media Content

Volume : 8 / No. : 2 / Pub. : 2016-11
  1. Macroscopical and microscopical analysis on the gonad of bombay duck fish (harpadon nehereus) caught in ujung pangkah and weru waters, east java
  2. The addition of papain on commercial feed to growth rate, feed conversion ratio and survival rate of eel fish (anguillla bicolor) stadia elver
  3. Silage substitution chemically solid waste surimi fish swanggi (priacanthus macracanthus) on to retention protein fish meal and retention fat tilapia (oreochromis niloticus)
  4. Anatomic pathology of comet fish (carassius auratus auratus) as result of males and females argulus japonicus infestation in different degree
  5. Activity enzymatic of isolate lactic acid bacteria from the digestive tract of mud crab (scylla spp.) as a candidate probiotics
  6. Chitosan as alternative to preservative kamaboko fish kurisi (nemipterus nematophorus) at cold temperature storage