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Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 7 / No. 2 / Published : 2015-01

TOC : 3, and page :133 - 140

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Original Article :

Potential of bacillus licheniformis and streptomyces olivaceoviridis as inhibiting the growth of fungus saprolegnia sp, cause saprolegniasis on fish by using in vitro

Author :

  1. Hari Suprapto*1
  2. Rahayu Kusdarwati*2
  3. Oktantia Frenny Anggani*3
  1. Dosen Fakultas Perairan dan Kelautan
  2. Dosen Fakultas Perairan dan Kelautan
  3. Mahasiswa Fakultas Perairan dan Kelautan

Abstract :

Saprolegniasis is a fungal disease on fish caused by Saprolegnia sp, which is saprophyte, damaging healthy tissue and makes the immune system in fish deacreased. The uniquennes of Saprolegnia sp has the main components of the cell wall in the form of chitin that was instrumental in shaping the structure of the tip growth of fungal hyphae. Control of fungal pathogenic Saprolegnia sp can use chitinolytic microorganisms based on ability to produce chitinase for example using bacteria.   This study aims to potential of Bacillus licheniformis and Streptomyces olivaceoviridis as inhibiting the growth of fungus Saprolegnia sp, cause saprolegniasis on fish by using in vitro. The research method is experimental with completely randomized design (CRD), which consists of  four treatments with five  replications. The experimental used is A (Saprolegnia sp and Bacillus licheniformis), B (Saprolegnia sp and Streptomyces olivaceoviridis), C negative control (Saprolegnia sp) and D positive control (Saprolegnia sp and Ketokonazol 2 %). The main parameters measured were observed inhibition zone on each treatment. Supporting parameters were observed is an observation of abnormal hpyphae structure after being induced by bacteria Bacillus licheniformis dan Streptomyces olivaceoviridis. Data were analyzed using analysis of variants (ANOVA) and to know the difference between  treatments were determined by Tukey honestly significant difference (Tukey HSD) Test.  The results showed that the potential of chitinolytic Bacillus licheniformis can provide a good barrier of 4,62 cm by 5,48 cm compared Streptomyces olivaceoviridis in inhibiting the growth Saprolegnia sp. Suggestions in this research is the need to further research on the value of chitinase Bacillus licheniformis and Streptomyces olivaceoviridis. Further research by using in vivo. 

Keyword :

Bacillus licheniformis, Streptomyces olivaceoviridis, Saprolegnia sp, Inhibition Zone,


References :

  1. A. Ratnaningtyas , (2013). Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaemferia galanga L.) terhadap Saprolegnia sp secara in vitro. 8 9-10 : Program Studi Budidaya Perairan : Fakultas Perikanan dan Kelautan Universitas Airlangga Surabaya
  2. Kusriningrum, (2010). Perancangan Percobaan. hal 10 : Airlangga University Press
  3. W. Haliza ; M. T. Suhartono, (2012). Karakteristik Kitinase dari Mikroba. 8 (1) : 1-14 : Institut Pertanian Bogor


   


Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2015-01
  1. Papaya leaf powder potential to improve efficiency utilization of feed, protein efficiency ratio and relative growth rate in tilapia (oreochromis niloticus) fish farming
  2. Characterization of edible film from propagules mangrove lindur (bruguiera gymnorrhiza) starch with addition of carboxymethyl cellulose (cmc) as plasticizer
  3. Potential of bacillus licheniformis and streptomyces olivaceoviridis as inhibiting the growth of fungus saprolegnia sp, cause saprolegniasis on fish by using in vitro
  4. The comparison of viscosity, flash point and freeze point biodiesel of seagrass (eucheuma denticulatum), sardinella oil (sardinellla longiceps) and commercial biodiesel
  5. Viral nervous necrosis (vnn) examination at fish by polymerase chain reaction (pcr) method
  6. The basic histology technique of gouramy fish (osphronemus gourami)
  7. The influence of papaya seed’s (carica papaya) toward the damage argulus japonicus’s eggs
  8. Content of crude protein and crude fiber in peanut leaves (arachis hypogaea) which is fermented by enterobacter cloacae wpl 111 as alternative fish feed stuff
  9. Financial analysis of fish enlargement milkfish (chanos chanos) in pond with traditional farming and monoculture polyculture system in mulyorejo, surabaya, east java
  10. Chemical silage substitution of swanggi surimi solid waste (priacanthus macracanthus) of fish meal toward energy retention and tiplapia seeds (oreochromis niloticus) feed conversion rate
  11. The influence of fixer for the quality of coloring biopigmen eucheuma sp. as a subtitute for synthetic dyes in textiles
  12. The influence of additional red pepper (capsicum annuum) juice in fish feed to koi fish's (cyprinus carpio l.) color brightness level
  13. Effect of nesting site depth of green turtle (chelonia mydas) on the incubation period and hatching success percentage in sukamade beach, meru betiri national park, banyuwangi in east java
  14. Subtitution of fish mill with shark waste mill (carcharhinus sp) on growth, feed efficiency and fish survival rate of african catfish (clarias sp.)
  15. The effect of addition probiotic plus herbal on commercial feed to protein retention and fat retention red tilapia fish (oreochromis niloticus)
  16. Effect of extract sargassum sp. with methanol solvent in feed on erythrocythes and differential leucocythes of african catfish (clarias gariepinus)
  17. Hematologic changes comet goldfish (carassius auratus auratus) as a result of infestation argulus japonicus males and argulus japonicus females
  18. Haematology and histopatology of gills, intestine and brain koi fish (cyprinus carpio koi) myxobolus koi orally infected