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Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 5 / No. 2 / Published : 2013-11

TOC : 16, and page :211 - 215

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Original Article :

The potential antagonistic bacterium lactobacillus plantarum against bacterial pathogens aeromonas salmonicida by in vitro

Author :

  1. Yunifar Amad*1
  2. Rahayu Kusdarwati*2
  3. Gunanti Mahasri *3
  1. Mahasiswa Fakultas Perairan dan Kelautan
  2. Dosen Fakultas Perairan dan Kelautan
  3. Dosen Fakultas Perairan dan Kelautan

Abstract :

Problems were often experienced by fish farmers is bacterial pathogen Aeromonas salmonicida which causes  furunculosis disease  in fish. The way to control  the growth of this pathogenic bacteria was using antagonist bacteria of Lactobacillus plantarum. Growth of L. plantarum may inhibit contamination of pathogenic bacterial because of its ability to produce bacteriocins, produce lactic, moreover that  these bacteria can produce hydrogen  peroxide which can  function as an antibacterial. This  research aimed  to provide  information  on  the  use  of  bacterial  antagonists  L.  plantarum  in  inhibiting  the  growth  of pathogenic bacteria A. salmonicida on In Vitro. This research was conducted on October 2012 until January 2013 at dry laboratory in Fisheries and  Marine  Faculty  of  Airlangga  University  Surabaya.  This  research  used  Completely  Randomized Design (CRD) with five  treatments and four replications. Bacteria L. plantarum with 0 concentration for control (A), 106 (B), 107 (C), 108 (D), 109 (E) tested challenge by paper disc method with 106 concentrate of pathogenic bacteria A. salmonicida in vitro. The results showed  that L. plantarum with a concentration of 109 CFU/ml (E) was a  treatment that produced average of obstacle on the distribution of A. salmonicida amounted to 12,375 mm. Then the treatment with a concentration of 108 CFU/ml (D) with the average of obstacle 8,95 mm. While treatment with a concentration of 107 CFU/ml (C) and 106 CFU/ml (B) was having average of obstacle 6.8 mm and 6 mm was not  significantly  different  from  the control  (A) which had an average 6 mm  obstacle. So  it could  be  concluded  that  bacteria  L.  plantarum  had  antagonistic  potential  against  bacterial  pathogens A.salmonicida  which  indicated  by  the  obstacle  produced  by  L.  plantarum  on  growth  of  pathogenic bacteria A.salmonicida.  

Keyword :

Lactobacillus plantarum, Aeromonas salmonicida, paper disc test, obstacle ,


References :

  1. X, (2008). Petunjuk Praktikum Mikrobiologi Dasar. Purwokerto : Universitas Jendral Sudirman


   


Archive Article

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Volume : 5 / No. : 2 / Pub. : 2013-11
  1. Engineering technology fermentation waste seaweed as probiotics on the cultivation of intensive fish aquaponic system
  2. Potential use of red algae ethanol extract (kappaphycus alvarezii) as formalin substitute natural preservative in meat fish
  3. Potency analysis of sonneratia sp. at east coast surabaya through ecology and social economy studies
  4. Application of black soldier fly larval (hermetia illucens) as feed and artificial feed (pellets) for rainbow kurumoi fish (melanotaenia parva)
  5. Technologi engineering chitosan as a preservative and elevated levels of a protein on tofu
  6. Raffinose exploration cotton seeds lieu of formalin in preserving fish
  7. Substitution effect of artemia spp. with golden snail (pomacea canaliculata) and worm (lumbricus rubellus) on the growth and protein retention snakehead seed (channa striata)
  8. The granting of an enzyme lignosellulosa in feed artificial on the growth and the survival rate seed gourami (osphronemus gouramy lac.)
  9. Comparative study of ability nannochloropsis sp. and spirulina sp. as agent bioremediation of heavy metal plumbum (pb)
  10. Comparative study of ability nannochloropsis sp. and chlorella sp. as agent bioremediation of heavy metal plumbum (pb)
  11. Study of bioaccumulation heavy metal mercury (hg) on local sea cucumber (phyllophorus sp.) from surabaya east coast – east java
  12. Exploration the candidate probiotic bacteria in mangrove mud wonorejo
  13. Study of heavy metal lead (pb) in fish, crustaceans and mollusks at the northern coast of bangkalan, madura
  14. Measurements of extract ether digestibility, organic matter and energy feed in tilapia (oreochromis niloticus) using surgery techniques
  15. Subtitution of soybean meal with fermented lamtoro (leucaena glauca) on growth and feed efficiency of tilapia fish (oreochromis niloticus)
  16. The potential antagonistic bacterium lactobacillus plantarum against bacterial pathogens aeromonas salmonicida by in vitro
  17. Correlation of growth and carragenan content of kappaphycus alvarezii and eucheuma spinosum by different distance placement of floating rafts at the grujugan village, sub-district gapura, residence of sumenep