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Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 2 / No. 2 / Published : 2007-08

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Original Article :

The production of superoxide from shrimp penaeus monodon hemocyte as an stress indicator on the intensive culture

Author :

  1. Boedi Setya Rahardja*1
  2. Hari Suprapto*2
  3. Muhajir*3
  1. Program Studi Perikanan, Universitas Airlangga
    Jl. Mulyorejo Kampus C Surabaya 60115
  2. Program Studi Perikanan, Universitas Airlangga
    Jl. Mulyorejo Kampus C Surabaya 60115
  3. Program Studi Perikanan, Universitas Airlangga
    Jl. Mulyorejo Kampus C Surabaya 60115

Abstract :

The experiments were deal to understand the production of superoxide from shrimp hemocyte P.monodon after and before stimulated with Vibrio bacterin zymosan and ECP. The shrimp hemocyte have a phagocytic activity against bacteria or other foreign substance, but the activity will disturbed if shrimp under stress. The production of superoxide was low if shrimp reared under stress condition compared to shrimp reared under normal condition. The OD value hemocyte of shrimp reared under heavy density induced by zymosan is 0.52, Vibrio sp 0.46 and ECP 0.45. Meanwhile OD value shrimp reared without stress condition are 1.2 induced by zymosan, Vibrio sp. 1.19 and ECP 1.18. The OD vale of control fish are 0.61 induce by zymosan, 0.6 Vibrio sp. bacterin and ECP the lowest of 0.55. PI value of phagocytic hemocyte was relatively low after 1 and 3 days stress treatment are 2.8 and 1.9 while the control are 1.5 and 1.3. The low value of PI due to the decreased of phagocytic function of hemocyte. Based on the results,the production of superoxide is influenced by the stress, therefore to keep the shrimp under low stress is very good aquaculture management.

Keyword :

superoxide, shrimp hemocyte, stress, intensive culture,


References :

  1. N. Bodhipaksha, (1994). The effects of methyl parathion on phagocytosis and respiratory burst activity of Tiger shrimp P. monodon phagocytes. In “Modulator of fish immune response” Eds by. J.S.Stolen and F.C. Fletcher. Fair Haven. USA. : SOS Publication
  2. Weeks-Perkin B.A., (1994). The effects of methyl parathion on phagocytosis and respiratory burst activity of Tiger shrimp P. monodon phagocytes. In “Modulator of fish immune response” Eds by. J.S.Stolen and F.C. Fletcher. Fair Haven. USA. : SOS Publication
  3. F.K. Higson, (1984). The generation of active oxygen species by stimulated rainbow trout leucocyte in whole blood. : 583 - 587. . Fair Haven. USA. : Comp. Biochem. Physiol. 77 B
  4. Jones O.T.G., (1984). The generation of active oxygen species by stimulated rainbow trout leucocyte in whole blood. : 583 - 587. . Fair Haven. USA. : Comp. Biochem. Physiol. 77 B
  5. K. Kawai, (1981). Mechanisms for protection in ayu orally vaccinated for vibriosis. - : Fish.Pathology, 15
  6. Kusuda R., (1981). Mechanisms for protection in ayu orally vaccinated for vibriosis. - : Fish.Pathology, 15
  7. Itami T., (1981). Mechanisms for protection in ayu orally vaccinated for vibriosis. - : Fish.Pathology, 15
  8. D.B. Lowrie, (1985). Activation of macrophage for antimicrobial activity. - : Immunology Letter, 11
  9. Jackett P.S., (1985). Activation of macrophage for antimicrobial activity. - : Immunology Letter, 11
  10. Andrew P.W., (1985). Activation of macrophage for antimicrobial activity. - : Immunology Letter, 11
  11. A.D. Pickering, (1990). Stress and the suppression of somatic growth in teleost. In Progress in Comparative Immunology. Ed. by Epple, A., C.G. Scannes and M.H. Stetson . - : -
  12. Y. Takahasi, (1995). Immunodefense system of crustacea. : 0. . - : Fish Pathology, 30
  13. Itami Y., (1995). Immunodefense system of crustacea. : 0. . - : Fish Pathology, 30
  14. Kondo M., (1995). Immunodefense system of crustacea. : 0. . - : Fish Pathology, 30


   


Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2010-11
  1. The use of sand substrate as media for tiger shrimp (penaeus monodon) post larva rearing
  2. The production of superoxide from shrimp penaeus monodon hemocyte as an stress indicator on the intensive culture
  3. Isolation of aeromonas hydrophila from diseased shrimp penaeus monodon cultured in traditional ponds
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