Catalog :




Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 5 / No. 1 / Published : 2013-04

Related with : Scholar   Yahoo!   Bing

Original Article :

Uji aktivitas antifungi ekstrak daun sirih (piper betle l) terhadap saprolegnia sp secara in vitro

Author :

  1. Rahayu Kusdarwati*1
  2. Pustika Murtinintias*2
  3. Dewa Ketut Meles*3
  1. Program Studi Budidaya Perairan Fakultas Perikanan dan Kelautan UNAIR
  2. Program Studi Budidaya Perairan Fakultas Perikanan dan Kelautan UNAIR
  3. Program Studi Budidaya Perairan Fakultas Perikanan dan Kelautan UNAIR

Abstract :

Saprolegniasis is a mycotic disease caused by Saprolegnia sp. that usually attacking wild fish and farming fish. Saprolegnia sp. cause a lot of harm in process of the fish cultivation. Prevention and treatment of the common practice is use chemical drugs, but the use of these chemicals tend to be environmentally unfriendly and there are has karsinogenik effect. Therefore, the use of traditional medicines is one of alternative to control Saprolegnia sp. safer than chemical drugs. Green betel leaf contains phenolic compounds and tannins are efficacious as antifungal agent.This study aims to prove the antifungal activity of extracts of betel leaf (Piper betle L) for Saprolegnia sp., and to know the minimum concentration of betel leaf extract (Piper betle L) as antifungal for Saprolegnia sp. The design of this experiment is used completely randomized design (CRD) with 11 treatments and 3 replications. This study used the dilution method through the Minimum Inhibitory Concentration determination (MIC) and Minimum Fungicidal Concentration (MFC). The concentration of the extract used was 50% (0.5 g/ml), 25% (0.25 g/ml), 12.5% (0.125 g/ml), 6.25% (0.0625 g/ml), 3.13% (0.0313 g/ml), 1.56% (0.0156 g/ml), 0.78% (0.0078 g/ml), 0.39% (0.0039 g/ml), 0.2% (0.002 g/ml) of betel leaf extract. A positive control containing 2 ml of 3% hydrogen peroxide were added fungal suspension until 4 ml Negative control containing 2 ml of 10% DMSO were added fungal suspension until 4 ml.The main parameters in this study is the value of optical density (OD) for MIC (Minimum Inhibitory Concentration) test and the absence of Saprolegnia sp. growing on SDA media for MFC (Minimum Fungicidal Concentration) test. 0.20% (0.002g/ml) concentration of of betel leaf extract is the minimum concentration that can inhibit the growth of Saprolegnia sp. MFC test results showed concentrations of 0.78% (0.0078 g/ml) betel leaf extract is the minimum concentration that can kill Saprolegnia sp.

Keyword :

betel leaf, Saprolegnia sp.,


References :

  1. J. Akbar, (2010). Uji Efektivitas Ekstrak Bawang Dayak (Eleutherine Palmifolia Merr) Terhadap Penyembuhan Infeksi Jamur Saprolegnia Sp, Pada Ikan Nila.. Banjarbaru : Jurusan Budi Daya Perairan, Fakultas Perikanan Dan Ilmu Kelautan Universitas Lambung Mangkurat.
  2. I. Nurswida, (2002). Efektivitas Dekok Sirih Hijau dan Sirih Kuning Dalam Menghambat Pertumbuhan Candida albicans (uji In Vitro).. Malang : Universitas Brawijaya
  3. D.Trifena, (2007). Aktifitas Kitinase Dan Sifat Antijamur Actinomycetes, Serratia Marcescens Serta Getah Pohon Karet. Bandung. : Skripsi. Fakultas Matematika Dan Ilmu Pengetahuan Alam. Institut Tekhnologi Bandung
  4. P.T.K. Woo, (1999). Fish Disease And Disorder Volume 3 Viral Bacterial And Fungal Infection. New York : CABI Publishing
  5. E. Jawetz, (1998). Farmakologi dasar dan klinik. Ed VI.. Jakarta. : Farmakologi dasar dan klinik. Ed VI


   


Archive Article

Cover Media Content

Volume : 5 / No. : 1 / Pub. : 2013-04
  1. Analisis kondisi kualitas air pada budidaya ikan kerapu tikus (cromileptes altivelis) di situbondo
  2. Studi kualitas air pada pembesaran ikan mas koki (carassius auratus) di sukabumi
  3. Pengaruh biofilter rumput laut gracilaria sp. terhadap dominansi plankton pada media air yang terpapar logam berat cr
  4. Uji aktivitas antifungi ekstrak daun sirih (piper betle l) terhadap saprolegnia sp secara in vitro
  5. Uji aktivitas antifungi ekstrak rimpang kencur (kaempferia galanga l.) terhadap saprolegnia sp. secara in vitro
  6. Substitusi bungkil kedelai dengan ddgs (distillers dried grains and solubles) terhadap kandungan lemak kasar dan energi daging ikan nila merah (oreochromis niloticus)
  7. Studi kandungan logam berat kadmium (cd) pada spesies ikan kembung (rastrelliger kanagurta) dan kerang darah (anadara granosa) di perairan manyar, gresik dan di perairan jabon, sidoarjo
  8. Identifikasi dan prevalensi cacing pada saluran pencernaan ikan gurami (osphronemus gouramy) di desa ngrajek magelang jawa tengah
  9. Distribusi penyakit infectious myo necrosis virus (imnv) pada udang vannamei (litopenaeus vannamei) di pantai utara jawa timur
  10. Pengaruh medium yang tercemar organoklorin (endosulfan) terhadap kandungan agar dan morfologi thallus gracilaria verrucosa
  11. Influence use of bandotan (ageratum conyzoides) to rate koi fish (cyprinus carpio) blood glucose after transportation
  12. Fermentation of coconut dregs using trichoderma viride, bacillus subtilis, and em4 against crude protein and crude fiber as an alternative feed ingredients for fish
  13. Effect of giving nata de nanno and nannochloropsis oculata on population of brachionus plicatilis
  14. Effect of iodized feeding on the growth and content of iodine in carpfish seed (cyprinus carpio)
  15. Effect of photoperiod and salinity on the growth and chlorophyll a from sargassum sp.
  16. Effect of different live feed type (skeletonema sp., chaetosceros sp., tetraselmis sp.) to the growth rate and nutritional content on artemia sp.
  17. The prevalence of ectoparasits that attack the seed of koi fish in the stock of ornamental fish surabaya
  18. Effect of lemna minor fertilizer concentration on dunaliella salina population
  19. Pengukuran daerah genangan di pesisir bangkalan akibat naiknya muka air laut