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Jurnal Oftalmologi Indonesia

ISSN 1693-2587

Vol. 5 / No. 3 / Published : 2007-12

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Original Article :

Comparison of p53 expression in hpv(+) and hpv(-) in conjunctival and eyelid squamous neoplasia

Author :

  1. Agus Supartoto*1
  2. Syenny Budi Handoko*2
  1. Department of Ophthalmology Gadjah Mada University, Sardjito Hospital Yogyakarta
  2. Department of Ophthalmology Gadjah Mada University, Sardjito Hospital Yogyakarta

Abstract :

Purpose :To compare the expression of p53 in HPV(+) and HPV (-) in neoplasia squamous cell of the eyelid and ocular surface. Design: A consecutive retrospective comparative study. Methods: All samples were taken from the paraffin block of squamous cell neoplasia that arouse on the eyelid and ocular surface that were operated between 2000-2007 in Sardjito General Hospital and Yap Eye Hospital. All samples were deparaffinized and cut in slices. Then it was stained using DO-7 humanized monoclonal antibody to observe the expression of p53 and the presence of HPV was detected by mouse monoclonal antibody K1H8 staining. Result: There were 15 samples that were included in this study. Seven samples were taken from the eyelid and 8 samples were taken from conjunctiva or fornix. Seven samples were HPV(+), and the mean expression of p53 was 29.7. Four of them were eyelid tumors and 3 of them were conjunctival tumors. Eight samples were HPV(-) and the mean expression of p53 was 26.5, three of them were eyelid tumors, and 5 were conjunctival tumors. The difference of p53 expression between the two groups was 3.24 with p value=0.791. Conclusion: The roles of p53 gene and HPV are important factors to be considered, regarding the malignancy and high recurrence rate of squamous cell neoplasia in eyelid and ocular surface. It is hoped that in the future there will be vaccine that can prevent the development of squamous cell neoplasia in high risk individuals.

Keyword :

neoplasia squamous cell, eyelid, conjunctiva, p53, HPV,


References :



       


    Archive Article

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