Detail Article

Jurnal Oftalmologi Indonesia

ISSN 1693-2587

Vol. 7 / No. 2 / Published : 2009-12

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Original Article :

The difference between total and active transforming growth factor e2 concentration on fresh and freeze-dried amnion membrane (laboratory experimental study in dr. soetomo general hospital surabaya)

Author :

  1. Elisabeth Srisubekti*1
  2. Diany Yogiantoro*2
  3. Gatut Suhendro*3
  1. Department of Ophthalmology, Faculty of Medicine Airlangga University/Dr. Soetomo General Hospital, Surabaya

Abstract :

The aim of this study was to evaluate the change of Transforming Growth FactorE2 (TGF-E2) concentration due to freeze-driedmethod. This study was an experimental laboratory study, performed on 14 amniotic membranes at biomaterial centre/tissue bankingDr. Soetomo General Hospital Surabaya. Each amniotic membrane was divided into two parts. The first part was extracted in freshand the second part underwent freeze-dried preparation before extracted. Both parts of the amniotic membrane were extracted usingultrasonic disintegrator. Concentration of TGF-E2 with and without activation was measured using ELISA method. The averageconcentration of TGF-E2 without activation (active) in fresh amniotic membrane was 274 ± 264,9 pg/g and freeze-dried was 76,1± 97,6 pg/g, the average degradation of TGF-E2 was 43,2%. The average concentration of TGF-E2 with activation (total) in freshamniotic membrane was 445 ± 855,6 pg/g and freeze-dried was 347,4 ± 665pg/g. Active TGFE2 concentration in fresh amnioticmembrane is significantly higher than TGF-E2 concentration in freeze-dried amniotic membrane

Keyword :

freeze-dried amniotic membrane, TGFE2, ELISA,

References :

Pinnita Prabhasawat,,(1999) Application of Preserved Human Amniotic Membrane for Corneal Surface Reconstruction Bangkok : Deparment of Ophthalmology. Mahidol University

Patil Amil, Billore,(2006) Amnion Membrane Transaplantation A Fresh Tissue Experience - : Rotary Eye Institut, Navsari Gujarat

Virender Sangwan, Sanghamitra, Sushma, et al,(2007) Amniotic Amniotic Membrane Transplantation: A Review of Curent Indications in the Management of Ophthalmic Disorder - : www. Indian J Ophthalmol

Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2009-12
  1. The Comparison Of Active And Total Transforming Growth Factor-e2 Concentration On Fresh And Cryopreservation Amnion Membrane
  2. Aqueous Humortransforming Growth Factor-e2 Level In Patients With And Without Posterior Capsule Opacity After Congenital Cataract Surgery
  3. Surgical Result Of Pseudophakic Retinal Detachment In Cikini Hospital-school Of Medicine Christian University Of Indonesia Jakarta
  4. The Effectiveness Of Lipofilm Microemulsion Eye Drops In Dry Eye Syndrome By Enhancing The Tear Film Quality
  5. The Difference Between Total And Active Transforming Growth Factor E2 Concentration On Fresh And Freeze-dried Amnion Membrane (laboratory Experimental Study In Dr. Soetomo General Hospital Surabaya)
  6. Management Of Upper Eyelid Coloboma With Three Steps Technique Surgery
  7. Bilateral Papil Atrophy In A Case With Intracerebellar Tuberculoma