Jurnal Oftalmologi Indonesia
ISSN 1693-2587
Vol. 7 / No. 2 / Published : 2009-12
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Original Article :
The difference between total and active transforming growth factor e2 concentration on fresh and freeze-dried amnion membrane (laboratory experimental study in dr. soetomo general hospital surabaya)
Author :
- Elisabeth Srisubekti*1
- Diany Yogiantoro*2
- Gatut Suhendro*3
- Department of Ophthalmology, Faculty of Medicine Airlangga University/Dr. Soetomo General Hospital, Surabaya
Abstract :
The aim of this study was to evaluate the change of Transforming Growth FactorE2 (TGF-E2) concentration due to freeze-driedmethod. This study was an experimental laboratory study, performed on 14 amniotic membranes at biomaterial centre/tissue bankingDr. Soetomo General Hospital Surabaya. Each amniotic membrane was divided into two parts. The first part was extracted in freshand the second part underwent freeze-dried preparation before extracted. Both parts of the amniotic membrane were extracted usingultrasonic disintegrator. Concentration of TGF-E2 with and without activation was measured using ELISA method. The averageconcentration of TGF-E2 without activation (active) in fresh amniotic membrane was 274 ± 264,9 pg/g and freeze-dried was 76,1± 97,6 pg/g, the average degradation of TGF-E2 was 43,2%. The average concentration of TGF-E2 with activation (total) in freshamniotic membrane was 445 ± 855,6 pg/g and freeze-dried was 347,4 ± 665pg/g. Active TGFE2 concentration in fresh amnioticmembrane is significantly higher than TGF-E2 concentration in freeze-dried amniotic membrane
Keyword :
freeze-dried amniotic membrane, TGFE2, ELISA,
References :
Pinnita Prabhasawat,,(1999) Application of Preserved Human Amniotic Membrane for Corneal Surface Reconstruction Bangkok : Deparment of Ophthalmology. Mahidol University
Patil Amil, Billore,(2006) Amnion Membrane Transaplantation A Fresh Tissue Experience - : Rotary Eye Institut, Navsari Gujarat
Virender Sangwan, Sanghamitra, Sushma, et al,(2007) Amniotic Amniotic Membrane Transplantation: A Review of Curent Indications in the Management of Ophthalmic Disorder - : www. Indian J Ophthalmol
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