Detail Article

Jurnal Oftalmologi Indonesia

ISSN 1693-2587

Vol. 5 / No. 3 / Published : 2001-01

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Original Article :

The difference of epidermal growth factor concentration between fresh and freeze-dried amniotic membranes

Author :

  1. Ihsan*1
  2. Prijanto *2
  1. Department of Ophthalmology, Faculty of Medicine Airlangga University/Dr. Soetomo General Hospital, Surabaya

Abstract :

This research has been conducted to measure the difference of Epidermal Growth Factor (EGF) concentration between freshand freeze-dried amniotic membranes. Experimental one group pretest - post test design was employed with 13 amniotic membranes.Each amniotic membrane was divided into two parts. The first part was without preservation (fresh) and the second part was withpreservation (freeze-dried). Both parts of amniotic membrane were extracted using ultrasonic disintegrator then the concentration ofEGF was measured using ELISA method. The result showed the average concentration of EGF in fresh amniotic membrane was 122,756± 11,591 pg/g while in freeze-dried amniotic membrane was 100,436 ± 9,690 pg/g. Average degradation of EGF concentration was22,320 ± 15,353 pg/g with estimated percentage degradation between 11,04–23,96%. It can be concluded that EGF concentration infresh amniotic membrane is significantly higher than EGF concentration in freeze-dried amniotic membrane.

Keyword :

EGF, fresh amniotic membrane, freeze-dried amniotic membrane, ELISA,

References :

Hennerbichler S, Reichl B, Pleiner D, et al,(2007) The Influence of Various Storage Conditions on Cell Viability in Amnion Membrane - : Cell Tissue Banking

Parolini, O., F. Alviano., G. P. Bagnara., et al,(2008) Concise Review: Concise Review: Isolation and Characterization of Cells from Human Term Placenta: Outcome of the First International Workshop on Placenta Derived Stem Cells - : STEMCELLS

Niknejad H, Habibollah P, Masoumeh J et al,(2008) Properties of the Properties of the Amniotic Membrane for Potential use in Tissue Engineering - : European cell and materials

Archive Article

Cover Media Content

Volume : 5 / No. : 3 / Pub. : 2008-12
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  13. Management Of The Complication After Silicone Fluid Injection At Facial And Eyelid Region
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