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Detail Article

Jurnal Penelitian Medika Eksakta

ISSN 1411-6626

Vol. 6 / No. 1 / Published : 2005-04

TOC : 3, and page :16 - 25

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Original Article :

Sitotoksisitas bahan restorasi cyanoacry- late dengan variasi perbandingan powder dan liquid menggunakan mtt assay

Author :

  1. Asti Meizarini*1
  2. Elly Munadziroh*2
  3. Prijawan Rachmadi*3
  1. Dosen Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi
  3. Dosen Fakultas Kedokteran Gigi

Abstract :

The requirements for dental material include should not toxic, not irritant, no carcinogenic potential, nor cause an allergic response with the use in oral cavity. The cyanoacrylate restoration material has certain substance that can be toxic. Because of the ratio amount of powder and liquid was not known, it can lead the restoration more toxic. The purpose of this study was to know the cytotoxicity of the cyanoacrylate restoration material with different variation of powder and liquid ratio using MTT assay. Six cylinder samples of 5 mm in diameter and 2 mm in thickness were use for each group of 1:1,00; 1:0,75; 1:0,50 powder and liquid ratio of cyanoacrylate restoration materials (Cyano Veneer-Meyer Haake). Each of sample were immersed in eppendorf microtubes consist of media culture. After 24 hour, the immersion of media culture were used to investigate the cytotoxic effect to BHK-21 cell lines by MTT assay method. The density of optic formazan was indicated the amount of living cells. All data were statistically analyzed by one-way Anova and HSD. The results showed that the amount of living cells of powder and liquid ratio 1:1,00; 1:0,75; 1:0,50 were 98,59%; 95,76; 94,92% respectively. There is a significant difference between 1:1,00 dengan 1:0,50 group ratio. The conclusion indicated that the cytotoxicity of 1:1,00; 1:0,75; 1:0,50 powder and liquid ratio of cyanoacrylate restoration materials in this study were decrease but not yet toxic.

Keyword :

Cytotoxicity, cyanoacrylate restoration material, powder and liquid ratio, MTT assay,


References :

  1. Rubianto, M., (1998). Biokompatibilitas Bahan Allograft (Human bone powder) dibandingkan dengan Bahan Alloplast (Hydroxylapatite). Surabaya : FKG UNAIR


   


Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2005-04
  1. Effect of the cucumber (cucumis sativus) as antioxidant toward cell membrane protection affecting cigarete smoke
  2. Fimbrial adhesin characterization of local streptococcus mutans playing a role the pathogenesis of dental caries
  3. Sitotoksisitas bahan restorasi cyanoacry- late dengan variasi perbandingan powder dan liquid menggunakan mtt assay