Catalog :




Detail Article

Journal of Parasite Science

ISSN -

Vol. 2 / No. 1 / Published : 2018-03

TOC : 5, and page :21 - 28

Related with : Scholar   Yahoo!   Bing

Original Article :

Anthelmintic activity ethanol extract of ocimum sanctum linn. leaves against ascaridia galli in vitro

Author :

  1. Vanna Lidya Kharisma*1
  2. Setiawan Koesdarto*2
  3. Koesnoto Supriandono*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aims of this research are to determine concentration, exposure time, interaction between concentration and exposure time of ethanol extract of Ocimum sanctum Linn. Leaves to cause death toward Ascaridia galli in vitro, and the value of LC50 and LC90 ethanol extract of Ocimum sanctum Linn. Leaves. Research design that has been used in the research was completely randomized design. This research used 200 samples of Ascaridia galli with length 7-11 cm without differentiating their sex. The concentration ethanol extract of Ocimum sanctum Linn. leaves were 1.25%, 2.5%, 5%, 10%. The control was using CMC-Na 0.5%. Each treatment then being replicated four times. The observation and recording of dead worm were done at 0, 3, 6, 12 and 24 hours. Ascaridia galli were declared dead if there was no movement while disturbed by anatomy tweezers and dipped in slightly warm water (50ºC). The obtained data was analyzed using Anova Factorial and continued with Duncan Multiple Range Test by SPSS for Windows 22. The result were 10% concentration and exposure time for 24 hours caused the most mortality toward Ascaridia galli. Interaction between concentration and exposure time resulted 10% concentration ethanol extract of Ocimum sanctum Linn. leaves in 24 hours caused the most mortality towards Ascaridia galli. Probit analysis was used to calculate the LC50 and LC90 of Ocimum sanctum Linn. leaves. The results were LC50 ethanol extract of Ocimum sanctum Linn. leaves at 6 hours was 14.8%, at 12 hours was 4.8% and at 24 hours was 3.0% and the LC90 at 24 hours was 9.1%.

Keyword :

Ocimum sanctum Linn. leaves, Ascaridia galli, ethanol extract, in vitro,


References :

  1. Agarwal, C., N. L. Sharma and S.S. Gaurav, (2012). An Analysis of Basil (Ocimum sp.) to Study The Morphological Variability. Vol. 3 No.3 Hal. 521-525. : Indian Journal of Fundamental and Applied Life Science
  2. Ali, S.A., G.E. Mohammed and A.A. Gamel, (2012). In vitro Adulticidal Efficacy of Albendazole, Capparis decidua Stems and Moringa oleifera Leaves against Fasciola gigantica. Vol. 13 No. 2 Hal. 59 - 67 : Sudan University of Science and Technology Journal of Science and Tech¬nology
  3. Andrew, R.W., C. Fryganas, A. Ramsay, Irene Mueller-Harvey, and M.T.Stig, (2014). Direct Anthelmintic Effects of Condensed Tannins from Diverse Plant Soures against Ascaris suum. Vol. 9 No. 5 : PLoS One


   


Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2018-03
  1. The prevalance and helminth infection degree of gastrointestinal in layer duck located in keper and markolak kramat village district of bangkalan regency of bangkalan
  2. The effect of calcium hydroxideon mortality of ascaridia galli in vitro
  3. The prevalance of gastrointestinal tract protozoa using fecal examination in local chicken(gallus domesticus) located in kramat village, district of bangkalan, bangkalan regency
  4. Prevalence and saverity level of scabies (sarcoptes scabiei) on rabbits in sajen village, pacet sub-district, mojokerto regency
  5. Anthelmintic activity ethanol extract of ocimum sanctum linn. leaves against ascaridia galli in vitro
  6. The conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit (lepus domesticus)
  7. Identification of worm in digestive tract of consumed cattle egret (bubulcus ibis) in ujung pangkah, gresik
  8. Efectiveness of ocimum sanctum linn. leaves ethanol extract on mortality raillietina echinobothrida in vitro
  9. Prevalence of nematodiasis in omasum and abomasum of goats slaughtered at pegirian surabaya slaughter house through gatrointestinal surgery method